Literature summary extracted from
Park, A.; Kim, I.; Do, H.; Jeon, B.; Lee, C.; Roh, S.; Shin, S.; Park, H.; Kim, Y.; Kim, Y.; Yoon, H.; Lee, J.; Kim, H.
Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica (2016), Sci. Rep., 6, 33903 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.6.5.4 |
- |
Oryza sativa Japonica Group |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.6.5.4 |
structures in the presence of cofactors, NAD+ and NADP+, and complexed with ascorbic acid and isoascorbic acid. The overall structure of MDHAR is similar to other iron-sulfur protein reductases, except for a unique long loop of 63-80 residues, which seems to be essential in forming the active site pocket. Residue Arg320 plays a major substrate binding role, and Tyr349 mediates electron transfer from NAD(P)H to bound substrate via FAD. The enzymatic activity favours NADH as an electron donor over NADPH |
Oryza sativa Japonica Group |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.6.5.4 |
C70A |
about 105% of wild-type activity |
Oryza sativa Japonica Group |
1.6.5.4 |
E196A |
residue E196 forms a hydrogen bond to the O2B molecule in the adenosine ring of NAD. Mutation reduces catalytic activity. Mutant E196A has only about 3 times higher affinity for NAD than that for NADP, i.e. 16fold increase in affinity for NADP compared to the wild-type |
Oryza sativa Japonica Group |
1.6.5.4 |
G72N |
about 70% of wild-type activity |
Oryza sativa Japonica Group |
1.6.5.4 |
R320A |
about 20% of wild-type activity |
Oryza sativa Japonica Group |
1.6.5.4 |
Y349A |
about 10% of wild-type activity |
Oryza sativa Japonica Group |
1.6.5.4 |
Y349F |
about 10% of wild-type activity |
Oryza sativa Japonica Group |
1.6.5.4 |
Y349W |
about 10% of wild-type activity |
Oryza sativa Japonica Group |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.6.5.4 |
Oryza sativa Japonica Group |
Q652L6 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.6.5.4 |
Os09g0567300 |
- |
Oryza sativa Japonica Group |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.6.5.4 |
NADH |
- |
Oryza sativa Japonica Group |
|
1.6.5.4 |
NADPH |
about 50% of the activity with NADH |
Oryza sativa Japonica Group |
|