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Literature summary extracted from

  • Li, G.; Yao, P.; Cong, P.; Ren, J.; Wang, L.; Feng, J.; Lau, P.C.; Wu, Q.; Zhu, D.
    New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates (2016), Sci. Rep., 6, 24973 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.12 expressed in Escherichia coli Top10 cells Microbacterium oxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.12 L199F the mutant is more active than the wild type enzyme toward the primary amines Microbacterium oxydans
1.4.3.12 L199I the mutant is more active than the wild type enzyme toward the primary amines Microbacterium oxydans
1.4.3.12 L199T the mutation enhances the enzyme activity toward the secondary amines Microbacterium oxydans
1.4.3.12 M226A the mutant exhibits enhanced activity relative to the wild type enzyme for most (S)-enantiomers of primary amines and some secondary amines Microbacterium oxydans
1.4.3.12 M226I the mutant is more active than the wild type enzyme toward the primary amines Microbacterium oxydans
1.4.3.12 M226T the mutant is more active than the wild type enzyme toward the primary amines Microbacterium oxydans
1.4.3.12 T198A the mutant exhibits enhanced activity relative to the wild type enzyme for most (S)-enantiomers of primary amines and some secondary amines Microbacterium oxydans
1.4.3.12 T198F the mutation enhances the enzyme activity toward the secondary amines Microbacterium oxydans
1.4.3.12 T198F/L199S/M226F the substrate 2-methyl-1, 2, 3, 4-tetrahydroquinoline is deracemized by the triple mutant Microbacterium oxydans
1.4.3.12 T198I the mutant is more active than the wild type enzyme toward the primary amines Microbacterium oxydans
1.4.3.12 Y321A the mutation enhances the enzyme activity toward the secondary amines Microbacterium oxydans
1.4.3.12 Y321F the mutation enhances the enzyme activity toward the secondary amines Microbacterium oxydans
1.4.3.12 Y321I the mutation enhances the enzyme activity toward the secondary amines and displays an enhanced catalytic efficiency toward 1-(4-methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline Microbacterium oxydans
1.4.3.12 Y321I/M226T the double mutant acts on (S)-N-(prop-2-yn-1-yl)-2,3-dihydro-1H-inden-1-amine Microbacterium oxydans
1.4.3.12 Y321T the mutation enhances the enzyme activity toward the secondary amines Microbacterium oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.12 cyclohexylamine + O2 + H2O Microbacterium oxydans
-
 cyclohexanone + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.12 Microbacterium oxydans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.12 (1R)-1-(4-chlorophenyl)ethan-1-amine + H2O + O2
-
 Microbacterium oxydans 1-(4-chlorophenyl)ethanone + NH3 + H2O2
-
 ?
1.4.3.12 (1R)-1-phenylethan-1-amine + H2O + O2
-
 Microbacterium oxydans 1-phenylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.12 (1S)-1-(4-chlorophenyl)ethan-1-amine + H2O + O2
-
 Microbacterium oxydans 1-(4-chlorophenyl)ethanone + NH3 + H2O2
-
 ?
1.4.3.12 (1S)-1-phenylethan-1-amine + H2O + O2
-
 Microbacterium oxydans 1-phenylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.12 (1S)-1-phenylpropan-1-amine + H2O + O2
-
 Microbacterium oxydans 1-phenylpropan-1-one + NH3 + H2O2
-
 ?
1.4.3.12 (2R)-heptan-2-amine + H2O + O2
-
 Microbacterium oxydans heptan-2-one + NH3 + H2O2
-
 ?
1.4.3.12 (2S)-heptan-2-amine + H2O + O2
-
 Microbacterium oxydans heptan-2-one + NH3 + H2O2
-
 ?
1.4.3.12 (S)-N-benzyl-1-phenylethylamine + O2 + H2O
-
 Microbacterium oxydans ?
-
 ?
1.4.3.12 cyclohexylamine + O2 + H2O
-
 Microbacterium oxydans cyclohexanone + NH3 + H2O2
-
 ?
1.4.3.12 additional information no activitiy towards (R)-N-(prop-2-yn-1-yl)-2, 3-dihydro-1H-inden-1-amine, 1-(4-methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline, (S)-N-methyl-1-phenylethanamine, and 1-methyl-1,2,3,4-tetrahydroisoquinoline Microbacterium oxydans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.12 CHAO
-
 Microbacterium oxydans