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Literature summary extracted from

  • Eppinger, E.; Stolz, A.
    Expansion of the substrate range of the gentisate 1,2-dioxygenase from Corynebacterium glutamicum for the conversion of monohydroxylated benzoates (2017), Protein Eng. Des. Sel., 30, 57-65 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.4 A112D the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum
1.13.11.4 A112G the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum
1.13.11.4 A112I the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum
1.13.11.4 A112S the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 salicylate
-
 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.13
-
 2,5-Dihydroxybenzoate at pH 8.0 and 25°C Corynebacterium glutamicum
1.13.11.4 0.99
-
 5-Fluorosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum
1.13.11.4 2.7
-
 5-Aminosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2 Corynebacterium glutamicum the enzyme is highly specific for 2,5-dihydroxybenzoate maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 Corynebacterium glutamicum ATCC 13032 the enzyme is highly specific for 2,5-dihydroxybenzoate maleylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Corynebacterium glutamicum Q8NLB9
-
-
1.13.11.4 Corynebacterium glutamicum ATCC 13032 Q8NLB9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4 Ni-NTA column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2 the enzyme is highly specific for 2,5-dihydroxybenzoate Corynebacterium glutamicum maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 the enzyme is highly specific for 2,5-dihydroxybenzoate Corynebacterium glutamicum ATCC 13032 maleylpyruvate
-
 ?
1.13.11.4 5-aminosalicylate + O2
-
 Corynebacterium glutamicum ?
-
 ?
1.13.11.4 5-aminosalicylate + O2
-
 Corynebacterium glutamicum ATCC 13032 ?
-
 ?
1.13.11.4 5-fluorosalicylate + O2
-
 Corynebacterium glutamicum ?
-
 ?
1.13.11.4 5-fluorosalicylate + O2
-
 Corynebacterium glutamicum ATCC 13032 ?
-
 ?
1.13.11.4 additional information no activity with salicylate, 3-methylsalicylate, 4-methylsalicylate, 5-methylsalicylate, 3-bromosalicylate, 4-bromosalicylate, 5-iodosalicylate, and 1-hydroxy-2-naphthoate Corynebacterium glutamicum ?
-
 ?
1.13.11.4 additional information no activity with salicylate, 3-methylsalicylate, 4-methylsalicylate, 5-methylsalicylate, 3-bromosalicylate, 4-bromosalicylate, 5-iodosalicylate, and 1-hydroxy-2-naphthoate Corynebacterium glutamicum ATCC 13032 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.4 GDO
-
 Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.4 0.4
-
 5-Fluorosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum
1.13.11.4 1.1
-
 5-Aminosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum
1.13.11.4 2310
-
 2,5-Dihydroxybenzoate at pH 8.0 and 25°C Corynebacterium glutamicum