Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Campos, P.; Levin, L.; Wirth, S.
    Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463 (2016), Process Biochem., 51, 895-903 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents Coriolopsis trogii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression of lcc3 gene in Komagataella (i.e. Pichia) pastoris Coriolopsis trogii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.25
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4, 30°C Coriolopsis trogii
1.10.3.2 2.095
-
 2,6-dimethoxyphenol pH 4, 30°C Coriolopsis trogii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Coriolopsis trogii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ multicopper enzyme Coriolopsis trogii

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Coriolopsis trogii A0A140CWW4
-
-
1.10.3.2 Coriolopsis trogii BAFC 463 A0A140CWW4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coriolopsis trogii BAFC 463 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Coriolopsis trogii ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Coriolopsis trogii BAFC 463 ?
-
 ?
1.10.3.2 additional information the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C Coriolopsis trogii ?
-
 ?
1.10.3.2 additional information the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C Coriolopsis trogii BAFC 463 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Coriolopsis trogii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 40 75 maximum activity at 50°C, retaining more than 80% of activity at temperatures between 60°C and 75°C, but less than 55% of activity at temperatures below 40°C Coriolopsis trogii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
 the enzyme retains 100% of its initial activity after 3 h of incubation Coriolopsis trogii
1.10.3.2 70
-
 half-life: 45 min Coriolopsis trogii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 329
-
 2,6-dimethoxyphenol pH 4, 30°C Coriolopsis trogii
1.10.3.2 399
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4, 30°C Coriolopsis trogii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Coriolopsis trogii
1.10.3.2 4
-
 substrate: 2,6-dimethoxyphenol Coriolopsis trogii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6 7 25°C, no decrease of activity for at least 5 days Coriolopsis trogii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 157
-
 2,6-dimethoxyphenol pH 4, 30°C Coriolopsis trogii
1.10.3.2 1596
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4, 30°C Coriolopsis trogii