EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.4 | expression in Escherichia coli | Aspergillus niger |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.4 | C141S | reductive amination activity of C141S alone is insensitive to treatment with 2-hydroxyethyl disulfide | Aspergillus niger |
1.4.1.4 | C415S | mutant loses its reductive amination activity in a manner very similar to the native enzyme | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | 2-hydroxyethyl disulfide | attenuation of reductive amination (forward) activity but a negligible effect on oxidative deamination (reverse) activity | Aspergillus niger | |
1.4.1.4 | cystine | strongly and selectively inhibits the reductive amination reaction | Aspergillus niger | |
1.4.1.4 | additional information | not inhibitory/activating: oxidized glutathione | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.4 | 0.011 | - |
NADPH | wild-type, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 0.017 | - |
NADP+ | wild-type, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 0.022 | - |
NADP+ | wild-type inactivated by 2-hydroxyethyl disulfide, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 0.023 | - |
NADPH | recombinant wild-type, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 0.028 | - |
NADP+ | recombinant wild-type, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 0.044 | - |
NADPH | wild-type inactivated by 2-hydroxyethyl disulfide, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 1.05 | - |
NH3 | wild-type, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 1.1 | - |
NH3 | recombinant wild-type, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 1.2 | - |
NH3 | mutant C141S, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 2.6 | - |
NH3 | wild-type inactivated by 2-hydroxyethyl disulfide, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 4 | - |
2-oxoglutarate | mutant C141S, S0.5 value, Hill coefficient 2.3, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 4.78 | - |
2-oxoglutarate | wild-type, S0.5 value, Hill coefficient 3.2, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 5.7 | - |
2-oxoglutarate | recombinant wild-type, S0.5 value, Hill coefficient 3.5, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 15.5 | - |
2-oxoglutarate | mutant C415S, S0.5 value, Hill coefficient 4.9, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 25.1 | - |
2-oxoglutarate | wild-type inactivated by 2-hydroxyethyl disulfide, S0.5 value, Hill coefficient 2.4, pH 8.0, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 28.6 | - |
L-glutamate | mutant C141S, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 30.9 | - |
L-glutamate | recombinant wild-type, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 34.6 | - |
L-glutamate | wild-type, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 36.8 | - |
L-glutamate | mutant C415S, pH 9.3, temperature not specified in the publication | Aspergillus niger | |
1.4.1.4 | 49.8 | - |
L-glutamate | wild-type inactivated by 2-hydroxyethyl disulfide, S0.5 value, Hill coefficient 1.9, pH 9.3, temperature not specified in the publication | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.4 | Aspergillus niger | B6V7E4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + H2O + NADP+ | - |
Aspergillus niger | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? |