BRENDA - Enzyme Database show

Heterologous expression and biochemical characterization of two lipoxygenases in oriental melon, Cucumis melo var. makuwa Makino

Cao, S.; Chen, H.; Zhang, C.; Tang, Y.; Liu, J.; Qi, H.; PLoS ONE 11, e0153801 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.12
gene CmLOX10, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3); gene CmLOX13, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged truncated enzyme in Escherichia coli strain BL21(DE3)
Cucumis melo var. makuwa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.12
0.1258
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
0.1402
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
0.1566
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
0.2303
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.12
chloroplast
-
Cucumis melo var. makuwa
9507
-
1.13.11.12
plasma membrane
-
Cucumis melo var. makuwa
5886
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.12
linoleic acid + O2
Cucumis melo var. makuwa
-
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
?
1.13.11.12
linolenic acid + O2
Cucumis melo var. makuwa
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Cucumis melo var. makuwa
A0A125S6K7
cultivar Yumeiren
-
1.13.11.12
Cucumis melo var. makuwa
A0A125S6K8
cultivar Yumeiren
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.12
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography; recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Cucumis melo var. makuwa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
linoleic acid + O2
-
743601
Cucumis melo var. makuwa
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
-
?
1.13.11.12
linolenic acid + O2
-
743601
Cucumis melo var. makuwa
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.12
?
x * 102301, sequence calculation; x * 102312, sequence calculation
Cucumis melo var. makuwa
1.13.11.12
More
enzyme domain structure, overview; enzyme domain structure, overview
Cucumis melo var. makuwa
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.12
35
-
-
Cucumis melo var. makuwa
1.13.11.12
45
-
-
Cucumis melo var. makuwa
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.12
20
50
enzyme activity of CmLOX10 increases as temperature rises between 20°C and 45°C and then decreases at 50°C, profile overview; enzyme activity of CmLOX13 is sharply decreased when the temperature is higher than 35°C, profile overview
Cucumis melo var. makuwa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.12
1841.7
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
2438.6
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
2442.96
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
5492.88
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
5
-
-
Cucumis melo var. makuwa
1.13.11.12
5.5
-
-
Cucumis melo var. makuwa
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.13.11.12
Cucumis melo var. makuwa
sequence calculation
-
6.1
1.13.11.12
Cucumis melo var. makuwa
sequence calculation
-
6.34
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
gene CmLOX10, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)
Cucumis melo var. makuwa
1.13.11.12
gene CmLOX13, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged truncated enzyme in Escherichia coli strain BL21(DE3)
Cucumis melo var. makuwa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.12
0.1258
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
0.1402
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
0.1566
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
0.2303
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.12
chloroplast
-
Cucumis melo var. makuwa
9507
-
1.13.11.12
plasma membrane
-
Cucumis melo var. makuwa
5886
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.12
linoleic acid + O2
Cucumis melo var. makuwa
-
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
?
1.13.11.12
linolenic acid + O2
Cucumis melo var. makuwa
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Cucumis melo var. makuwa
1.13.11.12
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Cucumis melo var. makuwa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
linoleic acid + O2
-
743601
Cucumis melo var. makuwa
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
-
?
1.13.11.12
linolenic acid + O2
-
743601
Cucumis melo var. makuwa
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.12
?
x * 102301, sequence calculation
Cucumis melo var. makuwa
1.13.11.12
?
x * 102312, sequence calculation
Cucumis melo var. makuwa
1.13.11.12
More
enzyme domain structure, overview
Cucumis melo var. makuwa
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.12
35
-
-
Cucumis melo var. makuwa
1.13.11.12
45
-
-
Cucumis melo var. makuwa
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.12
20
50
enzyme activity of CmLOX10 increases as temperature rises between 20°C and 45°C and then decreases at 50°C, profile overview
Cucumis melo var. makuwa
1.13.11.12
20
50
enzyme activity of CmLOX13 is sharply decreased when the temperature is higher than 35°C, profile overview
Cucumis melo var. makuwa
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.12
1841.7
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
2438.6
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
2442.96
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
5492.88
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
5
-
-
Cucumis melo var. makuwa
1.13.11.12
5.5
-
-
Cucumis melo var. makuwa
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.13.11.12
Cucumis melo var. makuwa
sequence calculation
-
6.1
1.13.11.12
Cucumis melo var. makuwa
sequence calculation
-
6.34
General Information
EC Number
General Information
Commentary
Organism
1.13.11.12
evolution
genes CmLOX10 and CmLOX13 include all of the typical LOX domains and share 58.11% identity at the amino acid level with each other. The phylogenetic analysis reveals that CmLOX10 and CmLOX13 are members of the type 2 13-LOX subgroup which are known to be involved in biotic and abiotic stress. The two lipoxygenases may play different functions in oriental melon during plant growth and development, CmLOX10 and CmLOX13 enzyme reaction shows that both enzymes produce 13S-hydroperoxides when linoleic acid is used as substrate; genes CmLOX10 and CmLOX13 include all of the typical LOX domains and share 58.11% identity at the amino acid level with each other. The phylogenetic analysis reveals that CmLOX10 and CmLOX13 are members of the type 2 13-LOX subgroup which are known to be involved in biotic and abiotic stress. The two lipoxygenases may play different functions in oriental melon during plant growth and development, CmLOX10 and CmLOX13 enzyme reaction shows that both enzymes produce 13S-hydroperoxides when linoleic acid is used as substrate
Cucumis melo var. makuwa
1.13.11.12
physiological function
the isozyme CmLOX10 is involved in biotic and abiotic stress; the isozyme CmLOX13 is involved in biotic and abiotic stress
Cucumis melo var. makuwa
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.12
evolution
genes CmLOX10 and CmLOX13 include all of the typical LOX domains and share 58.11% identity at the amino acid level with each other. The phylogenetic analysis reveals that CmLOX10 and CmLOX13 are members of the type 2 13-LOX subgroup which are known to be involved in biotic and abiotic stress. The two lipoxygenases may play different functions in oriental melon during plant growth and development, CmLOX10 and CmLOX13 enzyme reaction shows that both enzymes produce 13S-hydroperoxides when linoleic acid is used as substrate
Cucumis melo var. makuwa
1.13.11.12
physiological function
the isozyme CmLOX10 is involved in biotic and abiotic stress
Cucumis melo var. makuwa
1.13.11.12
physiological function
the isozyme CmLOX13 is involved in biotic and abiotic stress
Cucumis melo var. makuwa
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.13.11.12
11760
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
17420
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
19390
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
23850
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.13.11.12
11760
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
17420
-
linolenic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
19390
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa
1.13.11.12
23850
-
linoleic acid
pH 5.0, 45°C, recombinant Hi-tagged enzyme
Cucumis melo var. makuwa