Any feedback?
Literature summary extracted from
- Biochemical and physiological analyses of NADPH-dependent thioredoxin reductase isozymes in Euglena gracilis (2015), Plant Sci., 236, 29-36 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | - |
Euglena gracilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | additional information | expression as a truncated form without both the plastid-targetingpeptide and Trx domain | Euglena gracilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.0056 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis |  |
| 1.8.1.9 | 0.0224 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 0.0247 | - |
NADPH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 0.0806 | - |
NADH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 2.4 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 2.5 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 11.9 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.1.9 | cytosol | - |
Euglena gracilis | 5829 | - |
| 1.8.1.9 | mitochondrion | - |
Euglena gracilis | 5739 | - |
| 1.8.1.9 | plastid | - |
Euglena gracilis | 9536 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Euglena gracilis | W8VNN5 | isoform Ntr1 | - |
| 1.8.1.9 | Euglena gracilis | W8VVL6 | isoform NtrC | - |
| 1.8.1.9 | Euglena gracilis | W8VZ54 | isoform Ntr2 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | proteolytic modification | sequence contains a putative very short mitochondria-targeting siglal peptide | Euglena gracilis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 0.55 | - |
truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis |
| 1.8.1.9 | 0.92 | - |
pH 7.2, temperature not specified in the publication | Euglena gracilis |
| 1.8.1.9 | 7.32 | - |
pH 7.2, temperature not specified in the publication | Euglena gracilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | 5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+ | - |
Euglena gracilis | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
| 1.8.1.9 | 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Euglena gracilis | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | ? | x *34600, calculated | Euglena gracilis |
| 1.8.1.9 | ? | x *51800, calculated | Euglena gracilis |
| 1.8.1.9 | ? | x *53200, calculated | Euglena gracilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | Ntr1 | - |
Euglena gracilis |
| 1.8.1.9 | NTR2 | - |
Euglena gracilis |
| 1.8.1.9 | Ntr3 | - |
Euglena gracilis |
| 1.8.1.9 | NTRC | - |
Euglena gracilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.2 | - |
NADH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 0.59 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 0.88 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 0.97 | - |
NADPH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 1.23 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 6.4 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 10.7 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | additional information | no cofactor: NADH | Euglena gracilis | |
| 1.8.1.9 | NADH | about 1% of the catalytic effciency with NADPH, truncated mutant | Euglena gracilis | |
| 1.8.1.9 | NADH | about 2.5% of the activity with NADPH | Euglena gracilis | |
| 1.8.1.9 | NADPH | - |
Euglena gracilis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | physiological function | growth rate of isoform Ntr1 mutants is significantly lower than that of control cells | Euglena gracilis |
| 1.8.1.9 | physiological function | growth rate of isoform Ntr2 mutants is significantly lower than that of control cells | Euglena gracilis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.1 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 0.38 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 2.48 | - |
NADH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 4.33 | - |
5,5'-dithio-bis(2-nitrobenzoic acid) | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 26.5 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 398.5 | - |
NADPH | truncated mutant, pH 7.2, temperature not specified in the publication | Euglena gracilis | |
| 1.8.1.9 | 1220 | - |
NADPH | pH 7.2, temperature not specified in the publication | Euglena gracilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
