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Literature summary extracted from
- Glutathione reductase a unique enzyme molecular cloning, expression and biochemical characterization from the stress adapted C4 plant, Pennisetum glaucum (L.) R. Br (2015), Mol. Biol. Rep., 42, 947-962 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | expression in Escherichai coli | Cenchrus americanus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | homology modeling based on Trypanosoma brucei TPR protein, PDB ID 2WBA | Cenchrus americanus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.1449 | - |
NADPH | pH 8.0, temperature not specified in the publication | Cenchrus americanus |  |
| 1.8.1.7 | 0.1538 | - |
glutathione disulfide | pH 8.0, temperature not specified in the publication | Cenchrus americanus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Cenchrus americanus | A0A068EUE1 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | glutathione disulfide + NADPH + H+ | - |
Cenchrus americanus | 2 glutathione + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | ? | x * 53500, calculated, x * 53000, SDS-PAGE | Cenchrus americanus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 40 | - |
- |
Cenchrus americanus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 70 | - |
about 50% of maximum activity | Cenchrus americanus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 8 | - |
- |
Cenchrus americanus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 6 | 10 | - |
Cenchrus americanus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | NADPH | - |
Cenchrus americanus | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.8.1.7 | Cenchrus americanus | transcript level is downregulated by 0.5fold following 1 h of salt stress | down |
| 1.8.1.7 | Cenchrus americanus | transcript is significantly upregulated in response to salt and methyl viologen | up |
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