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Literature summary extracted from

  • Mueller, J.E.; Meyer, F.; Litsanov, B.; Kiefer, P.; Potthoff, E.; Heux, S.; Quax, W.J.; Wendisch, V.F.; Brautaset, T.; Portais, J.C.; Vorholt, J.A.
    Engineering Escherichia coli for methanol conversion (2015), Metab. Eng., 28, 190-201 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus coagulans Adh is increased by the endogenous activator protein Act, a Nudix hydrolase Weizmannia coagulans
1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, no activation in vivo Bacillus methanolicus
1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh3 is increased by the endogenous activator protein Act, a Nudix hydrolase Bacillus methanolicus
1.1.1.244 Act activator protein, UniProt ID I3EA59, the in vitro activity of Desulfitobacterium hafniense Adh is increased by the endogenous activator protein Act, a Nudix hydrolase Desulfitobacterium hafniense
1.1.1.244 Act protein activator protein, UniProt ID I3EA59, the in vitro activity of Lysinibacillus sphaericus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase Lysinibacillus sphaericus
1.1.1.244 additional information no activation by activator protein Act, UniProt ID I3EA59 Lysinibacillus fusiformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.244 gene BFZC1_05383, recombinant expression in Escherichia coli Lysinibacillus fusiformis
1.1.1.244 gene Bsph_4187, recombinant expression in Escherichia coli Lysinibacillus sphaericus
1.1.1.244 gene mdh, recombinant expression in Escherichia coli Bacillus methanolicus
1.1.1.244 gene mdh1, recombinant expression in Escherichia coli Bacillus methanolicus
1.1.1.244 gene mdh2, recombinant expression in Escherichia coli Bacillus methanolicus
1.1.1.244 gene mdh3, recombinant expression in Escherichia coli Bacillus methanolicus
1.1.1.244 recombinant enzyme expression in Escherichia coli Weizmannia coagulans
1.1.1.244 recombinant enzyme expression in Escherichia coli Desulfitobacterium hafniense
1.1.1.244 recombinant enzyme expression in Escherichia coli Desulfofundulus kuznetsovii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.244 D38G site-directed mutagenesis, the mutant shows altered activity compared to the wild-type enzyme Bacillus methanolicus
1.1.1.244 D41G site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Bacillus methanolicus
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview Weizmannia coagulans
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview Desulfitobacterium hafniense
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview Lysinibacillus fusiformis
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview Bacillus methanolicus
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview Lysinibacillus sphaericus
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), system evaluation, overview Desulfofundulus kuznetsovii
1.1.1.244 additional information utilization of methanol in Escherichia coli is allowed by the implementation of NAD-dependent methanol dehydrogenase and the establishment of the ribulose monophosphate cycle by expressing the genes for hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), up to 40% incorporation of methanol into central metabolites is achieved, system evaluation, overview Bacillus methanolicus
1.1.1.244 S97G site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Bacillus methanolicus
1.1.1.244 S97G site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Bacillus methanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.244 methanol + NAD+ Weizmannia coagulans
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Desulfitobacterium hafniense
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Lysinibacillus fusiformis
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Bacillus methanolicus
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Lysinibacillus sphaericus
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Desulfofundulus kuznetsovii
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Desulfofundulus kuznetsovii MGA3
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Lysinibacillus fusiformis ZC1
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Desulfitobacterium hafniense Y51
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Bacillus methanolicus PB1
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Weizmannia coagulans 36D1
-
 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+ Bacillus methanolicus MGA3
-
 formaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.244 Bacillus methanolicus I3DTM5
-
-
1.1.1.244 Bacillus methanolicus I3DTP7
-
-
1.1.1.244 Bacillus methanolicus I3DVX6
-
-
1.1.1.244 Bacillus methanolicus I3DX19
-
-
1.1.1.244 Bacillus methanolicus I3E2P9
-
-
1.1.1.244 Bacillus methanolicus I3E949
-
-
1.1.1.244 Bacillus methanolicus MGA3 I3DTM5
-
-
1.1.1.244 Bacillus methanolicus MGA3 I3E2P9
-
-
1.1.1.244 Bacillus methanolicus MGA3 I3E949
-
-
1.1.1.244 Bacillus methanolicus PB1 I3DTP7
-
-
1.1.1.244 Bacillus methanolicus PB1 I3DVX6
-
-
1.1.1.244 Bacillus methanolicus PB1 I3DX19
-
-
1.1.1.244 Desulfitobacterium hafniense
-
-
-
1.1.1.244 Desulfitobacterium hafniense Y51
-
-
-
1.1.1.244 Desulfofundulus kuznetsovii
-
-
-
1.1.1.244 Desulfofundulus kuznetsovii MGA3
-
-
-
1.1.1.244 Lysinibacillus fusiformis
-
-
-
1.1.1.244 Lysinibacillus fusiformis ZC1
-
-
-
1.1.1.244 Lysinibacillus sphaericus B1HX72
-
-
1.1.1.244 Weizmannia coagulans
-
-
-
1.1.1.244 Weizmannia coagulans 36D1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.244 additional information
-
 no activity of the recombinant enzyme in Escherichia coli suspension cells Lysinibacillus fusiformis
1.1.1.244 additional information
-
 no activity of the recombinant enzyme in Escherichia coli suspension cells Bacillus methanolicus
1.1.1.244 0.0007
-
 recombinant wild-type Mdh in Escherichia coli suspension cells, pH 7.4, 37°C, with addition of Act Bacillus methanolicus
1.1.1.244 0.0007
-
 recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.001
-
 recombinant wild-type Adh in Escherichia coli suspension cells, pH 7.4, 37°C Lysinibacillus sphaericus
1.1.1.244 0.0014
-
 recombinant wild-type Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0015
-
 recombinant wild-type Mdh3 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0017
-
 recombinant wild-type Mdh in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0018
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Desulfitobacterium hafniense
1.1.1.244 0.0023
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0026
-
 recombinant wild-type Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0029
-
 recombinant wild-type Adh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Lysinibacillus sphaericus
1.1.1.244 0.003
-
 recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C Weizmannia coagulans
1.1.1.244 0.003
-
 recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0033
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0038
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Lysinibacillus fusiformis
1.1.1.244 0.0038
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Lysinibacillus fusiformis
1.1.1.244 0.0051
-
 recombinant mutant S97G Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0057
-
 recombinant mutant S97G Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0058
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Weizmannia coagulans
1.1.1.244 0.007
-
 recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C Desulfitobacterium hafniense
1.1.1.244 0.008 4 recombinant wild-type Adh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Lysinibacillus sphaericus
1.1.1.244 0.01
-
 recombinant wild-type Mdh1 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0114
-
 recombinant mutant S97G Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.014
-
 recombinant mutant S97G Mdh in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0155
-
 recombinant wild-type Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Bacillus methanolicus
1.1.1.244 0.0155
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Weizmannia coagulans
1.1.1.244 0.0167
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Desulfitobacterium hafniense
1.1.1.244 0.021
-
 recombinant mutant S97G Mdh in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Bacillus methanolicus
1.1.1.244 0.0213
-
 recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C, with addition of Act Bacillus methanolicus
1.1.1.244 0.023
-
 recombinant wild-type Mdh3 in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.0273
-
 recombinant wild-type Mdh3 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Bacillus methanolicus
1.1.1.244 0.03
-
 recombinant wild-type Mdh2 in Escherichia coli suspension cells, pH 7.4, 37°C Bacillus methanolicus
1.1.1.244 0.045
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 7.4, 37°C, with addition of Act Bacillus methanolicus
1.1.1.244 0.22
-
 recombinant wild-type Mdh2 in Escherichia coli cell-free enzyme extract, pH 9.5, 45°C Bacillus methanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.244 methanol + NAD+
-
 Weizmannia coagulans formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Desulfitobacterium hafniense formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Lysinibacillus fusiformis formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Lysinibacillus sphaericus formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Desulfofundulus kuznetsovii formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Desulfofundulus kuznetsovii MGA3 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Lysinibacillus fusiformis ZC1 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Desulfitobacterium hafniense Y51 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Weizmannia coagulans 36D1 formaldehyde + NADH + H+
-
 r
1.1.1.244 methanol + NAD+
-
 Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.244 ADH
-
 Weizmannia coagulans
1.1.1.244 ADH
-
 Desulfitobacterium hafniense
1.1.1.244 ADH
-
 Lysinibacillus fusiformis
1.1.1.244 ADH
-
 Lysinibacillus sphaericus
1.1.1.244 ADH
-
 Desulfofundulus kuznetsovii
1.1.1.244 BFZC1_05383
-
 Lysinibacillus fusiformis
1.1.1.244 Bsph_4187
-
 Lysinibacillus sphaericus
1.1.1.244 MDH
-
 Bacillus methanolicus
1.1.1.244 Mdh1
-
 Bacillus methanolicus
1.1.1.244 MDH2
-
 Bacillus methanolicus
1.1.1.244 Mdh3
-
 Bacillus methanolicus
1.1.1.244 NAD-dependent methanol dehydrogenase
-
 Weizmannia coagulans
1.1.1.244 NAD-dependent methanol dehydrogenase
-
 Desulfitobacterium hafniense
1.1.1.244 NAD-dependent methanol dehydrogenase
-
 Lysinibacillus fusiformis
1.1.1.244 NAD-dependent methanol dehydrogenase
-
 Bacillus methanolicus
1.1.1.244 NAD-dependent methanol dehydrogenase
-
 Lysinibacillus sphaericus
1.1.1.244 NAD-dependent methanol dehydrogenase
-
 Desulfofundulus kuznetsovii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.244 37
-
 assay at Weizmannia coagulans
1.1.1.244 37
-
 assay at Desulfitobacterium hafniense
1.1.1.244 37
-
 assay at Lysinibacillus fusiformis
1.1.1.244 37
-
 assay at Bacillus methanolicus
1.1.1.244 37
-
 assay at Lysinibacillus sphaericus
1.1.1.244 37
-
 assay at Desulfofundulus kuznetsovii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.244 7.4
-
 assay at Weizmannia coagulans
1.1.1.244 7.4
-
 assay at Desulfitobacterium hafniense
1.1.1.244 7.4
-
 assay at Lysinibacillus fusiformis
1.1.1.244 7.4
-
 assay at Bacillus methanolicus
1.1.1.244 7.4
-
 assay at Lysinibacillus sphaericus
1.1.1.244 7.4
-
 assay at Desulfofundulus kuznetsovii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.244 NAD+
-
 Weizmannia coagulans
1.1.1.244 NAD+
-
 Desulfitobacterium hafniense
1.1.1.244 NAD+
-
 Lysinibacillus fusiformis
1.1.1.244 NAD+
-
 Bacillus methanolicus
1.1.1.244 NAD+
-
 Lysinibacillus sphaericus
1.1.1.244 NAD+
-
 Desulfofundulus kuznetsovii