BRENDA - Enzyme Database

A ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase with homologs in the domains Bacteria and Archaea

Yan, Z.; Wang, M.; Ferry, J.G.; mBio 8, e02285-16 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.7.3
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
1.8.98.1
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
1.8.98.4
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.7.3
0.05
-
CoM-S-S-CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.1
-
CoB
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.2
-
CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.2
-
CoM
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.4
-
CoM
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.1
-
coenzyme B
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.2
-
coenzyme B
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.2
-
coenzyme M
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.4
-
coenzyme M
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.7.3
Zn2+
required
Methanosarcina acetivorans
1.8.98.1
Zn2+
subunit HdrB2 contains zinc
Methanosarcina acetivorans
1.8.98.4
Zn2+
required
Methanosarcina acetivorans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.7.3
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
Methanosarcina acetivorans
-
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
r
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Methanosarcina acetivorans
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
r
1.8.98.4
oxidized coenzyme F420 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
Methanosarcina acetivorans
-
reduced coenzyme F420 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.7.3
Methanosarcina acetivorans
-
-
-
1.8.98.1
Methanosarcina acetivorans
-
-
-
1.8.98.4
Methanosarcina acetivorans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.7.3
-
Methanosarcina acetivorans
1.8.98.4
-
Methanosarcina acetivorans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.7.3
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
743210
Methanosarcina acetivorans
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
-
r
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
743210
Methanosarcina acetivorans
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
r
1.8.98.1
coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420
-
743210
Methanosarcina acetivorans
coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420
-
-
-
?
1.8.98.4
oxidized coenzyme F420 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
743210
Methanosarcina acetivorans
reduced coenzyme F420 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.7.3
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE
Methanosarcina acetivorans
1.8.98.1
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE; x * 87000, subunit HdrA2, SDS-PAGE
Methanosarcina acetivorans
1.8.98.4
?
x * 87000, subunit HdrA2, SDS-PAGE
Methanosarcina acetivorans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.7.3
[4Fe-4S]-center
-
Methanosarcina acetivorans
1.8.98.1
coenzyme F420
-
Methanosarcina acetivorans
1.8.98.1
FAD
-
Methanosarcina acetivorans
1.8.98.1
[2Fe-2S]-center
-
Methanosarcina acetivorans
1.8.98.1
[4Fe-4S]-center
-
Methanosarcina acetivorans
1.8.98.4
[4Fe-4S]-center
-
Methanosarcina acetivorans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.7.3
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
1.8.98.1
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
1.8.98.4
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.7.3
[4Fe-4S]-center
-
Methanosarcina acetivorans
1.8.98.1
coenzyme F420
-
Methanosarcina acetivorans
1.8.98.1
FAD
-
Methanosarcina acetivorans
1.8.98.1
[2Fe-2S]-center
-
Methanosarcina acetivorans
1.8.98.1
[4Fe-4S]-center
-
Methanosarcina acetivorans
1.8.98.4
[4Fe-4S]-center
-
Methanosarcina acetivorans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.7.3
0.05
-
CoM-S-S-CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.1
-
CoB
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.2
-
CoB
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.2
-
CoM
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.7.3
1.4
-
CoM
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.1
-
coenzyme B
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.2
-
coenzyme B
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.2
-
coenzyme M
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.8.98.1
1.4
-
coenzyme M
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.7.3
Zn2+
required
Methanosarcina acetivorans
1.8.98.1
Zn2+
subunit HdrB2 contains zinc
Methanosarcina acetivorans
1.8.98.4
Zn2+
required
Methanosarcina acetivorans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.7.3
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
Methanosarcina acetivorans
-
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
r
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Methanosarcina acetivorans
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
r
1.8.98.4
oxidized coenzyme F420 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
Methanosarcina acetivorans
-
reduced coenzyme F420 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.7.3
-
Methanosarcina acetivorans
1.8.98.4
-
Methanosarcina acetivorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.7.3
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
743210
Methanosarcina acetivorans
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
-
-
r
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
743210
Methanosarcina acetivorans
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
r
1.8.98.1
coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420
-
743210
Methanosarcina acetivorans
coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420
-
-
-
?
1.8.98.4
oxidized coenzyme F420 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
743210
Methanosarcina acetivorans
reduced coenzyme F420 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.7.3
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE
Methanosarcina acetivorans
1.8.98.1
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE; x * 87000, subunit HdrA2, SDS-PAGE
Methanosarcina acetivorans
1.8.98.4
?
x * 87000, subunit HdrA2, SDS-PAGE
Methanosarcina acetivorans