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Literature summary extracted from

  • Kochius, S.; Paetzold, M.; Scholz, A.; Merkens, H.; Vogel, A.; Ansorge-Schumacher, M.; Hollmann, F.; Schrader, J.; Holtmann, D.
    Enantioselective enzymatic synthesis of the alpha-hydroxy ketone (R)-acetoin from meso-2,3-butanediol (2014), J. Mol. Catal. B, 103, 61-66 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.B20 synthesis discovery of the (S)-selective alcohol dehydrogenase enables a novel production process of (R)-acetoin from meso-2,3-butanediol uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 ADH-9 gene is identified from a biodiversity library, recombinant expression in Escherichia coli strain BL21/(DE3) uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B20 additional information the regeneration of oxidised nicotinamide adenine dinucleotide is a key point in preparative application of dehydrogenases for the oxidative route. An electrochemical regeneration system is successfully combined with the BDH catalysed reaction. Up to 48 mM (R)-acetoin is produced in the reaction system while productivities up to 2 mM/h are reached. Possibility to apply an electrochemical system in a semi-preparative synthesis. Lyophilised recombinant ADH-9 from Escherichia coli BL21(DE3) cells is immobilized onto Amberlite FPA54 to 0.01 U per mg carrier leading to increased productivity compared to the immobilised form, method optimization uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.B20 soluble
-
 uncultured bacterium
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B20 meso-2,3-butanediol + NAD+ uncultured bacterium the (S)-specific ADH-9 produces (R)-acetoin by an oxidative route starting from meso-2,3-butanediol (R)-acetoin + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 uncultured bacterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B20 lyophilised recombinant ADH-9 from Escherichia coli BL21(DE3) cells by immobilization onto Amberlite FPA54 to 0.01 U per mg carrier uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B20 7.14
-
 crude recombinant enzyme, pH 7.0, 37°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 meso-2,3-butanediol + NAD+ the (S)-specific ADH-9 produces (R)-acetoin by an oxidative route starting from meso-2,3-butanediol uncultured bacterium (R)-acetoin + NADH + H+
-
 r
1.1.1.B20 meso-2,3-butanediol + NAD+ enantioselective enzymatic synthesis of the alpha-hydroxy ketone(R)-acetoin from meso-2,3-butanediol uncultured bacterium (R)-acetoin + NADH + H+
-
 r
1.1.1.B20 additional information reaction component identification and quantification by GC-MS. No or very poor activity with isopropanol 2-methyl-1-butanol, 2-methyl-2-butanol, 2,5-hexanediol, 1,4-butanediol uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 ADH-9
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B20 37
-
 assay at uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B20 7
-
 assay at uncultured bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NAD+
-
 uncultured bacterium
1.1.1.B20 NADH
-
 uncultured bacterium