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Literature summary extracted from

  • Widderich, N.; Pittelkow, M.; Höppner, A.; Mulnaes, D.; Buckel, W.; Gohlke, H.; Smits, S.; Bremer, E.
    Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase (2014), J. Mol. Biol., 426, 586-600 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.55 expressed in Escherichia coli DH5alpha cells Virgibacillus salexigens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.55 A163C the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 A163C/S244C the mutant shows increased activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 D148A inactive Virgibacillus salexigens
1.14.11.55 D148E inactive Virgibacillus salexigens
1.14.11.55 F143A inactive Virgibacillus salexigens
1.14.11.55 F143W inactive Virgibacillus salexigens
1.14.11.55 F143Y inactive Virgibacillus salexigens
1.14.11.55 F242A inactive Virgibacillus salexigens
1.14.11.55 F242W inactive Virgibacillus salexigens
1.14.11.55 F242Y the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 F263A inactive Virgibacillus salexigens
1.14.11.55 F263W inactive Virgibacillus salexigens
1.14.11.55 F263Y the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 F95A inactive Virgibacillus salexigens
1.14.11.55 H146A inactive Virgibacillus salexigens
1.14.11.55 H248A inactive Virgibacillus salexigens
1.14.11.55 N133A inactive Virgibacillus salexigens
1.14.11.55 N261A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 P198A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 Q129A inactive Virgibacillus salexigens
1.14.11.55 R131A inactive Virgibacillus salexigens
1.14.11.55 R259A inactive Virgibacillus salexigens
1.14.11.55 R259H inactive Virgibacillus salexigens
1.14.11.55 R259K inactive Virgibacillus salexigens
1.14.11.55 R259Q inactive Virgibacillus salexigens
1.14.11.55 S165A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 S167A the mutant shows about wild type activity Virgibacillus salexigens
1.14.11.55 S205A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 S244C the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 S250A inactive Virgibacillus salexigens
1.14.11.55 V265A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 V265L the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 V265T the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
1.14.11.55 W152A inactive Virgibacillus salexigens
1.14.11.55 W152F inactive Virgibacillus salexigens
1.14.11.55 W152Y inactive Virgibacillus salexigens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.55 4.5
-
 (4S)-ectoine mutant A163C/S244C, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 5.5
-
 (4S)-ectoine mutant N261A, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 5.9
-
 (4S)-ectoine wild type enzyme, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 6
-
 (4S)-ectoine mutant A163C, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 6.2
-
 (4S)-ectoine mutant S244C, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 6.8
-
 (4S)-ectoine mutant S205A, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 6.9
-
 (4S)-ectoine mutant S167A, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 7.1
-
 (4S)-ectoine mutant P198A, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 8
-
 (4S)-ectoine mutant F263Y, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 9.7
-
 (4S)-ectoine mutant V265T, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 16.2
-
 (4S)-ectoine mutant V265A, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 17.3
-
 (4S)-ectoine mutant S165A, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 19.6
-
 (4S)-ectoine mutant F242Y, at pH 7.5 and 32°C Virgibacillus salexigens
1.14.11.55 24.6
-
 (4S)-ectoine mutant V265L, at pH 7.5 and 32°C Virgibacillus salexigens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.55 Fe2+ required Virgibacillus salexigens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.55 (4S)-ectoine + 2-oxoglutarate + O2 Virgibacillus salexigens
-
 (4S,5S)-5-hydroxyectoine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.55 Virgibacillus salexigens Q2TDY4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.55 (4S)-ectoine + 2-oxoglutarate + O2
-
 Virgibacillus salexigens (4S,5S)-5-hydroxyectoine + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.55 ectD
-
 Virgibacillus salexigens