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Literature summary extracted from

  • Begara-Morales, J.C.; Sanchez-Calvo, B.; Chaki, M.; Mata-Perez, C.; Valderrama, R.; Padilla, M.N.; Lopez-Jaramillo, J.; Luque, F.; Corpas, F.J.; Barroso, J.B.
    Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation (2015), J. Exp. Bot., 66, 5983-5996 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.5.4 additional information presence of 0.5 or 2 mM glutathione does neither inhibit nor activate the enzyme Pisum sativum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 expression in Escherichia coli Pisum sativum

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.4 Y345F mutant is a functional enzyme resistant to inhibition by 0.5 mM and 5.0 mM peroxynitrite Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.4 additional information presence of 0.5 or 2 mM glutathione does neither inhibit nor activate the enzyme Pisum sativum
1.6.5.4 peroxynitrite exclusively nitrates residues Tyr213, Try292, and Tyr345. Tyr345 is found at 3.3 A of His313, which is involved in the NADP-binding site. Nitration of residue Tyr345 is responsible for inhibition Pisum sativum
1.6.5.4 S-nitrosoglutathione 0.5 and 2 mM, 58% and 65% inhibition, respectively Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 peroxisome
-
 Pisum sativum 5777
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Pisum sativum Q66PF9
-
-

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDHAR1
-
 Pisum sativum

Expression

EC Number Organism Comment Expression
1.6.5.4 Pisum sativum MDAR expression (mRNA, protein, and enzyme activity levels) increase upon treatment with 150 mM NaCl up