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Literature summary extracted from

  • Jin, J.; Kim, M.J.; Dhandapani, S.; Tjhang, J.G.; Yin, J.L.; Wong, L.; Sarojam, R.; Chua, N.H.; Jang, I.C.
    The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase (2015), J. Exp. Bot., 66, 3959-3975 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.81 gene TPS3, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method Cananga odorata var. fruticosa
4.2.3.115 gene TPS1, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, recombinant expression of YFP-tagged enzyme containing the N-terminal plastid Tp sequence in Nicotiana benthamiana leaf chloroplasts under control of CaMV 35S promoter via transformation method with Agrobacterium tumefaciens strain GV3101 Cananga odorata var. fruticosa
4.2.3.127 gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method Cananga odorata var. fruticosa
4.2.3.128 gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method Cananga odorata var. fruticosa
4.2.3.177 expression in Nicotiana benthamiana Cananga odorata var. fruticosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.81 cytosol
-
 Cananga odorata var. fruticosa 5829
-
4.2.3.115 chloroplast
-
 Cananga odorata var. fruticosa 9507
-
4.2.3.127 cytosol
-
 Cananga odorata var. fruticosa 5829
-
4.2.3.128 cytosol
-
 Cananga odorata var. fruticosa 5829
-
4.2.3.177 chloroplast
-
 Cananga odorata var. fruticosa 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.81 Mg2+ required, enzyme CoTPS3 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain Cananga odorata var. fruticosa
4.2.3.115 Mg2+ required, enzyme CoTPS1 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain Cananga odorata var. fruticosa
4.2.3.127 Mg2+ required, enzyme CoTPS2 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain Cananga odorata var. fruticosa
4.2.3.128 Mg2+ required, enzyme CoTPS2 has the conserved aspartate-rich motif (DDXXD) and NSE/DTE motifs that chelate divalent metal ions, typically Mg2+, in the C-terminal domain Cananga odorata var. fruticosa
4.2.3.177 Mg2+ assay in presence of 7.5 mM Cananga odorata var. fruticosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.81 (2E,6E)-farnesyl diphosphate Cananga odorata var. fruticosa
-
 (-)-exo-alpha-bergamotene + diphosphate
-
 ?
4.2.3.81 additional information Cananga odorata var. fruticosa enzyme CoTPS2 is multifunctional and novel TPS that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene ?
-
 ?
4.2.3.115 geranyl diphosphate Cananga odorata var. fruticosa
-
 alpha-terpinene + diphosphate
-
 ?
4.2.3.127 (2E,6E)-farnesyl diphosphate Cananga odorata var. fruticosa
-
 beta-copaene + diphosphate
-
 ?
4.2.3.127 additional information Cananga odorata var. fruticosa enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene ?
-
 ?
4.2.3.128 (2E,6E)-farnesyl diphosphate Cananga odorata var. fruticosa
-
 beta-cubebene + diphosphate
-
 ?
4.2.3.128 additional information Cananga odorata var. fruticosa enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene ?
-
 ?
4.2.3.177 geranyl diphosphate Cananga odorata var. fruticosa
-
 beta-thujene + diphosphate
-
 ?
4.2.3.177 additional information Cananga odorata var. fruticosa no substrate: farnesyl diphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.81 Cananga odorata var. fruticosa
-
 dwarf ylang ylang, grown in Singapore
-
4.2.3.115 Cananga odorata var. fruticosa
-
 dwarf ylang ylang, grown in Singapore
-
4.2.3.127 Cananga odorata var. fruticosa
-
 dwarf ylang ylang, grown in Singapore
-
4.2.3.128 Cananga odorata var. fruticosa
-
 dwarf ylang ylang, grown in Singapore
-
4.2.3.177 Cananga odorata var. fruticosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.81 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cananga odorata var. fruticosa
4.2.3.115 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cananga odorata var. fruticosa
4.2.3.127 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cananga odorata var. fruticosa
4.2.3.128 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cananga odorata var. fruticosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.81 flower
-
 Cananga odorata var. fruticosa
-
4.2.3.81 flower bud
-
 Cananga odorata var. fruticosa
-
4.2.3.81 leaf expression level of CoTPS3 is highest in mature green flowers Cananga odorata var. fruticosa
-
4.2.3.81 additional information the enzyme expression increases from flower buds, over undeveloped small flowers to mature green flowers, and decline in mature yellow flowers Cananga odorata var. fruticosa
-
4.2.3.115 flower mature yellow flower Cananga odorata var. fruticosa
-
4.2.3.115 additional information no alpha-terpinene production in flower bud, undeveloped small flowers, and mature green flowers Cananga odorata var. fruticosa
-
4.2.3.115 petal
-
 Cananga odorata var. fruticosa
-
4.2.3.127 flower
-
 Cananga odorata var. fruticosa
-
4.2.3.127 flower bud CoTPS2 is highly expressed at the floral bud stage Cananga odorata var. fruticosa
-
4.2.3.127 leaf very low expression level Cananga odorata var. fruticosa
-
4.2.3.127 additional information the enzyme expression increases from undeveloped small flowers to mature green flowers, is absent in mature yellow flowers, but very high in flower buds Cananga odorata var. fruticosa
-
4.2.3.128 flower
-
 Cananga odorata var. fruticosa
-
4.2.3.128 flower bud CoTPS2 is highly expressed at the floral bud stage Cananga odorata var. fruticosa
-
4.2.3.128 leaf very low expression level Cananga odorata var. fruticosa
-
4.2.3.128 additional information the enzyme expression increases from undeveloped small flowers to mature green flowers, is absent in mature yellow flowers, but very high in flower buds Cananga odorata var. fruticosa
-
4.2.3.177 flower highest expression in mature green flower Cananga odorata var. fruticosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.81 (2E,6E)-farnesyl diphosphate
-
 Cananga odorata var. fruticosa (-)-exo-alpha-bergamotene + diphosphate
-
 ?
4.2.3.81 additional information enzyme CoTPS2 is multifunctional and novel TPS that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa ?
-
 ?
4.2.3.115 geranyl diphosphate
-
 Cananga odorata var. fruticosa alpha-terpinene + diphosphate
-
 ?
4.2.3.127 (2E,6E)-farnesyl diphosphate
-
 Cananga odorata var. fruticosa beta-copaene + diphosphate
-
 ?
4.2.3.127 additional information enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa ?
-
 ?
4.2.3.128 (2E,6E)-farnesyl diphosphate
-
 Cananga odorata var. fruticosa beta-cubebene + diphosphate
-
 ?
4.2.3.128 additional information enzyme CoTPS2 is multifunctional and a terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa ?
-
 ?
4.2.3.177 geranyl diphosphate
-
 Cananga odorata var. fruticosa beta-thujene + diphosphate
-
 ?
4.2.3.177 additional information no substrate: farnesyl diphosphate Cananga odorata var. fruticosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.81 CoTPS2
-
 Cananga odorata var. fruticosa
4.2.3.81 TPS2
-
 Cananga odorata var. fruticosa
4.2.3.115 CoTPS1
-
 Cananga odorata var. fruticosa
4.2.3.115 TPS1
-
 Cananga odorata var. fruticosa
4.2.3.127 CoTPS2
-
 Cananga odorata var. fruticosa
4.2.3.127 TPS2
-
 Cananga odorata var. fruticosa
4.2.3.128 CoTPS2
-
 Cananga odorata var. fruticosa
4.2.3.128 TPS2
-
 Cananga odorata var. fruticosa
4.2.3.177 TPS1
-
 Cananga odorata var. fruticosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.81 30
-
 assay at Cananga odorata var. fruticosa
4.2.3.115 30
-
 assay at Cananga odorata var. fruticosa
4.2.3.127 30
-
 assay at Cananga odorata var. fruticosa
4.2.3.128 30
-
 assay at Cananga odorata var. fruticosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.81 7.4
-
 assay at Cananga odorata var. fruticosa
4.2.3.115 7.4
-
 assay at Cananga odorata var. fruticosa
4.2.3.127 7.4
-
 assay at Cananga odorata var. fruticosa
4.2.3.128 7.4
-
 assay at Cananga odorata var. fruticosa

General Information

EC Number General Information Comment Organism
4.2.3.81 evolution the enzyme belongs to the terpene sythase superfamily, subfamily TPS-b. The arginine-tryptophan motif, R(R)X8W, present at the N-terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS3 Cananga odorata var. fruticosa
4.2.3.81 metabolism the enzyme is part of the biosynthesis of volatile organic compounds in flowers Cananga odorata var. fruticosa
4.2.3.81 physiological function recombinant CoTPS3 catalysed the conversion of farnesyl pyrophosphate to alpha-bergamotene, from farnesyl diphosphate. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes Cananga odorata var. fruticosa
4.2.3.115 evolution the enzyme belongs to the terpene sythase superfamily, subfamily TPS-b. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS1, and CoTPS1 contains a putative N-terminal plastid Tp sequence of 41 amino acids Cananga odorata var. fruticosa
4.2.3.115 metabolism the enzyme is part of the biosynthesis of volatile organic compounds in flowers Cananga odorata var. fruticosa
4.2.3.115 physiological function the enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS1 synthesizes four products corresponding to beta-thujene, sabinene, beta-pinene, and -alpha from geranyl diphosphate Cananga odorata var. fruticosa
4.2.3.127 evolution the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence Cananga odorata var. fruticosa
4.2.3.127 metabolism the enzyme is part of the biosynthesis of volatile organic compounds in flowers Cananga odorata var. fruticosa
4.2.3.127 physiological function recombinant CoTPS2 catalyses the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa
4.2.3.128 evolution the enzyme belongs to the terpene sythase superfamily, subfamily TPS-a. The arginine-tryptophan motif, R(R)X8W, present at the N terminus of most mono-TPS and in some sesqui-TPS and di-TPS, is found in CoTPS2. CoTPS2 does not contain a plastid Tp sequence Cananga odorata var. fruticosa
4.2.3.128 metabolism the enzyme is part of the biosynthesis of volatile organic compounds in flowers Cananga odorata var. fruticosa
4.2.3.128 physiological function recombinant CoTPS2 catalysed the synthesis of three compounds, beta-ylangene, beta-copaene, and beta-cubebene, from farnesyl diphosphate. CoTPS2 is a multifunctional beta-ylangene/beta-copaene/beta-cubebene synthase capable of producing three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene. The enzyme is part of the biosynthesis of volatile organic compounds (VOCs) in flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. The scented flowers of Cananga odorata var. fruticosa are analysed by GC-MS and a total of 49 VOCs are identified at four different stages of flower development. The bulk of these VOCs are terpenes, mainly sesquiterpenes. Enzyme CoTPS2 is a multifunctional terpene synthase that catalyses the synthesis of three sesquiterpenes, beta-ylangene, beta-copaene, and beta-cubebene Cananga odorata var. fruticosa