EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.66 | 0.0053 | - |
NADPH | - |
Acinetobacter sp. | |
1.14.13.66 | 0.0062 | - |
2-Hydroxycyclohexan-1-one | - |
Acinetobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.63 | 158000 | - |
gel filtration | Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.174 | trans-cyclohexane-1,2-diol + NAD+ | Acinetobacter sp. | metabolic pathway of trans-cyclohexan-1,2-diol to adipate | 2-hydroxycyclohexan-1-one + NADH | - |
r | |
1.2.1.63 | 6-oxohexanoate + NADP+ + H2O | Nocardia globerula | - |
adipate + NADPH | - |
? | |
1.2.1.63 | 6-oxohexanoate + NADP+ + H2O | Acinetobacter sp. | - |
adipate + NADPH | - |
? | |
1.2.1.63 | 6-oxohexanoate + NADP+ + H2O | Nocardia globerula CL1 | - |
adipate + NADPH | - |
? | |
1.14.13.66 | 2-Hydroxycyclohexan-1-one + NADPH + O2 | Acinetobacter sp. | enzyme in the oxidation pathway of trans-cyclohexan-1,2-diol to adipate, enzyme activity is induced by trans-cyclohexan-1,2-diol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.174 | Acinetobacter sp. | - |
- |
- |
1.1.1.174 | Acinetobacter sp. TD63 | - |
- |
- |
1.2.1.63 | Acinetobacter sp. | - |
- |
- |
1.2.1.63 | Acinetobacter sp. NCIB 9871 | - |
- |
- |
1.2.1.63 | Acinetobacter sp. TD63 | - |
- |
- |
1.2.1.63 | Nocardia globerula | - |
- |
- |
1.2.1.63 | Nocardia globerula CL1 | - |
- |
- |
1.14.13.66 | Acinetobacter sp. | - |
TD63 | - |
1.14.13.66 | Acinetobacter sp. TD63 | - |
TD63 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.174 | partial | Acinetobacter sp. |
1.2.1.63 | partial | Acinetobacter sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.174 | culture condition:cyclohexan-1,2-diol grown cell | induction of enzyme formation by trans-cyclohexan-1,2-diol containing medium | Acinetobacter sp. | - |
1.2.1.63 | cell culture | - |
Nocardia globerula | - |
1.2.1.63 | cell culture | strain TD63 only inducible for enzyme synthesis in the presence of trans-cyclohexan-1,2-diol-containing growth media | Acinetobacter sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.174 | 0.85 | - |
cell extracts, grown on trans-cyclohexan-1,2-diol | Acinetobacter sp. |
1.1.1.174 | 26.5 | - |
partially purified | Acinetobacter sp. |
1.2.1.63 | 7.7 | - |
- |
Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.174 | trans-cyclohexane-1,2-diol + NAD+ | metabolic pathway of trans-cyclohexan-1,2-diol to adipate | Acinetobacter sp. | 2-hydroxycyclohexan-1-one + NADH | - |
r | |
1.2.1.63 | 6-oxohexanoate + NADP+ + H2O | - |
Nocardia globerula | adipate + NADPH | - |
? | |
1.2.1.63 | 6-oxohexanoate + NADP+ + H2O | - |
Acinetobacter sp. | adipate + NADPH | - |
? | |
1.2.1.63 | 6-oxohexanoate + NADP+ + H2O | - |
Nocardia globerula CL1 | adipate + NADPH | - |
? | |
1.14.13.66 | 2-hydroxycyclohexan-1-one + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-7-hydroxycycloheptane + NADP+ + H2O | 1-oxa-2-oxo-7-hydroxycycloheptane spontaneously rearranges to yield 6-oxohexanoate | ? | |
1.14.13.66 | 2-hydroxycyclohexan-1-one + NADPH + O2 | - |
Acinetobacter sp. TD63 | 1-oxa-2-oxo-7-hydroxycycloheptane + NADP+ + H2O | 1-oxa-2-oxo-7-hydroxycycloheptane spontaneously rearranges to yield 6-oxohexanoate | ? | |
1.14.13.66 | 2-hydroxycyclohexan-1-one + NADPH + O2 | - |
Acinetobacter sp. NCIB 9871 | 1-oxa-2-oxo-7-hydroxycycloheptane + NADP+ + H2O | 1-oxa-2-oxo-7-hydroxycycloheptane spontaneously rearranges to yield 6-oxohexanoate | ? | |
1.14.13.66 | 2-Hydroxycyclohexan-1-one + NADPH + O2 | enzyme in the oxidation pathway of trans-cyclohexan-1,2-diol to adipate, enzyme activity is induced by trans-cyclohexan-1,2-diol | Acinetobacter sp. | ? | - |
? | |
1.14.13.66 | 2-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
1.14.13.66 | 2-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. TD63 | ? | - |
? | |
1.14.13.66 | 2-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. NCIB 9871 | ? | - |
? | |
1.14.13.66 | 3-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
1.14.13.66 | 3-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. TD63 | ? | - |
? | |
1.14.13.66 | 3-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. NCIB 9871 | ? | - |
? | |
1.14.13.66 | 4-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
1.14.13.66 | 4-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. TD63 | ? | - |
? | |
1.14.13.66 | 4-Methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. NCIB 9871 | ? | - |
? | |
1.14.13.66 | Cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
1.14.13.66 | Cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. TD63 | ? | - |
? | |
1.14.13.66 | Cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. NCIB 9871 | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.174 | 7 | - |
reduction of 2-hydroxycyclohexan-1-one | Acinetobacter sp. |
1.1.1.174 | 9.5 | 10 | oxidation of trans-cyclohexane-1,2-diol | Acinetobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.174 | NAD+ | - |
Acinetobacter sp. | |
1.2.1.63 | NADP+ | - |
Acinetobacter sp. | |
1.14.13.66 | NADPH | - |
Acinetobacter sp. |