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Literature summary extracted from

  • Matsui, D.; Okazaki, S.; Matsuda, M.; Asano, Y.
    Enhancement of stability of L-tryptophan dehydrogenase from Nostoc punctiforme ATCC29133 and its application to L-tryptophan assay (2015), J. Biotechnol., 196-197, 27-32 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.19 expressed in Escherichia coli JM109 cells Nostoc punctiforme

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.19 A234D the mutant shows higher specific activity and stability compared to the wild type enzyme Nostoc punctiforme
1.4.1.19 D168G the mutant shows higher specific activity and stability compared to the wild type enzyme Nostoc punctiforme
1.4.1.19 I296N the mutant shows wild type specific activity and higher stability compared to the wild type enzyme Nostoc punctiforme
1.4.1.19 L59F the mutant shows higher specific activity and stability compared to the wild type enzyme Nostoc punctiforme
1.4.1.19 L59F/D168G/A234D/I296N mutant with thermal stability whose specific activity and stability are higher than those of the wild type enzyme Nostoc punctiforme

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.19 0.194
-
 L-tryptophan mutant enzyme L59F, at pH 11.5 and 30°C Nostoc punctiforme
1.4.1.19 0.201
-
 L-tryptophan wild type enzyme, at pH 11.5 and 30°C Nostoc punctiforme
1.4.1.19 0.221
-
 L-tryptophan mutant enzyme D168G, at pH 11.5 and 30°C Nostoc punctiforme
1.4.1.19 0.228
-
 L-tryptophan mutant enzyme I296N, at pH 11.5 and 30°C Nostoc punctiforme
1.4.1.19 0.268
-
 L-tryptophan mutant enzyme L59F/D168G/A234D/I296N, at pH 11.5 and 30°C Nostoc punctiforme
1.4.1.19 0.288
-
 L-tryptophan mutant enzyme A234D, at pH 11.5 and 30°C Nostoc punctiforme

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.19 (indol-3-yl)pyruvate + NH3 + NADH + H+ Nostoc punctiforme
-
 L-tryptophan + NAD+ + H2O
-
 r
1.4.1.19 (indol-3-yl)pyruvate + NH3 + NADH + H+ Nostoc punctiforme ATCC29133
-
 L-tryptophan + NAD+ + H2O
-
 r
1.4.1.19 L-tryptophan + NAD+ + H2O Nostoc punctiforme
-
 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
1.4.1.19 L-tryptophan + NAD+ + H2O Nostoc punctiforme ATCC29133
-
 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.19 Nostoc punctiforme W8CV45
-
-
1.4.1.19 Nostoc punctiforme ATCC29133 W8CV45
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.19 Ni-NTA column chromatography Nostoc punctiforme

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.19 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nostoc punctiforme L-tryptophan + NAD+ + H2O
-
 r
1.4.1.19 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nostoc punctiforme ATCC29133 L-tryptophan + NAD+ + H2O
-
 r
1.4.1.19 L-tryptophan + NAD+ + H2O
-
 Nostoc punctiforme (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
1.4.1.19 L-tryptophan + NAD+ + H2O
-
 Nostoc punctiforme ATCC29133 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.19 ? x * 40000, SDS-PAGE Nostoc punctiforme

Synonyms

EC Number Synonyms Comment Organism
1.4.1.19 NAD+-dependent L-tryptophan dehydrogenase
-
 Nostoc punctiforme
1.4.1.19 TrpDH
-
 Nostoc punctiforme

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.19 4
-
 the enzyme retains 19.3% activity after incubation at 4°C for 24 h Nostoc punctiforme

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.19 NAD+
-
 Nostoc punctiforme
1.4.1.19 NADH
-
 Nostoc punctiforme