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Literature summary extracted from

  • Huy, N.D.; Nguyen, C.L.; Park, H.S.; Loc, N.H.; Choi, M.S.; Kim, D.H.; Seo, J.W.; Park, S.M.
    Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium (2016), J. Biosci. Bioeng., 121, 154-159 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 degradation addition of recombinant Eg5A to cellobiase (cellobiohydrolase and beta-glucosidase) results in a 53% increase in saccharification of NaOH-pretreated barley straw, whereas the glucose release is 47% higher than with cellobiase treatment alone Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Pichia pastoris Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 additional information amino acids, E209 and E319, act as proton donor and nucleophile in the catalytic domain Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 0.0037
-
 carboxymethyl cellulose pH 5.0, 50°C Phanerodontia chrysosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Mn2+ 177% of initial activity in presence of Mn2+ Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Phanerodontia chrysosporium Q66NB7 endoglucanase family 5 protein
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 additional information sequence contains a carbohydrate binding module family 1 at the C-terminus, and one potential N-glycosylation site (Asn-Thr-Ser) is found Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 622
-
 substrate glucomannan, pH 5.0, 50°C Phanerodontia chrysosporium
3.2.1.4 694
-
 substrate carboxymethyl cellulose, pH 5.0, 50°C Phanerodontia chrysosporium
3.2.1.4 1444
-
 substrate beta-glucan, pH 5.0, 50°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O
-
 Phanerodontia chrysosporium cellotriose + ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.2.1.4 glucomannan + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.2.1.4 additional information very poor substrates: laminarin, xyloglucan, no substrates: birchwood xylan, arabinan, arabinoxylan, mannan, 4-nitrophenyl beta-D-glucopyranoside Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 43000, SDS-PAGE and calculated Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel5A
-
 Phanerodontia chrysosporium
3.2.1.4 Eg5a
-
 Phanerodontia chrysosporium
3.2.1.4 manganese dependent endoglucanase
-
 Phanerodontia chrysosporium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 20
-
 50% of maximum activity Phanerodontia chrysosporium
3.2.1.4 45 70
-
 Phanerodontia chrysosporium
3.2.1.4 80
-
 50% of maximum activity Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 stable for 12 h Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4
-
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 8
-
 Phanerodontia chrysosporium