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Literature summary extracted from

  • Huy, N.D.; Nguyen, C.L.; Seo, J.W.; Kim, D.H.; Park, S.M.
    Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase (2015), J. Biosci. Bioeng., 119, 416-420 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.37 degradation enzyme increases reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endoxylanase. The late addition of the enzyme into reaction with endoxylanase results in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endoxylanases alone. The increases observed are 6.3% and 13.8%, respectively Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Pichia pastoris Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Phanerodontia chrysosporium
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Phanerodontia chrysosporium W8QRE4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 25
-
 pH 5.5, 50°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 additional information no substrates: 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannoside, carboxymethylcellulose, barley glucan, glucomannan, laminarin. Very poor substrates: xyloglucan, locust bean gum, xylans. Enzyme can perform transglycosylation of xylose and alcohol Phanerodontia chrysosporium ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Phanerodontia chrysosporium 2 D-xylose
-
 ?
3.2.1.37 xylopentaose + 4 H2O
-
 Phanerodontia chrysosporium 5 D-xylose
-
 ?
3.2.1.37 xylotriose + 2 H2O
-
 Phanerodontia chrysosporium 3 D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 54000, SDS-PAGE of recombinant protein carrying an alpha secretion signal peptide Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 Xyl5
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 50
-
-
 Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 40
-
 stable up to Phanerodontia chrysosporium
3.2.1.37 50
-
 12 h, 50% residual activity Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5.5
-
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 5
-
 65% residual activity Phanerodontia chrysosporium
3.2.1.37 6
-
 80% residual activity Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 3 8 12 h, more than 80% residual activity Phanerodontia chrysosporium