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Literature summary extracted from

  • Isobe, K.; Taira, R.; Hoshi, Y.; Matsuda, S.; Yamada, M.; Hibi, M.; Kishino, S.; Ogawa, J.
    Characterization of a novel L-amino acid oxidase with protein oxidizing activity from Penicillium steckii AIU 027 (2014), J. Biosci. Bioeng., 117, 690-695 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 8-hydroxyquinoline 2% residual activity at 1 mM Penicillium steckii
1.4.3.2 alpha,alpha'-dipyridyl 15% residual activity at 1 mM Penicillium steckii
1.4.3.2 Co2+ 88% residual activity at 1 mM Penicillium steckii
1.4.3.2 EDTA 86% residual activity at 1 mM Penicillium steckii
1.4.3.2 Fe3+ complete inhibition at 1 mM Penicillium steckii
1.4.3.2 hydrazine 93% residual activity at 1 mM Penicillium steckii
1.4.3.2 hydroxylamine 12% residual activity at 1 mM Penicillium steckii
1.4.3.2 iodoacetate 75% residual activity at 1 mM Penicillium steckii
1.4.3.2 KCN 83% residual activity at 1 mM Penicillium steckii
1.4.3.2 Mg2+ 91% residual activity at 1 mM Penicillium steckii
1.4.3.2 Mn2+ 92% residual activity at 1 mM Penicillium steckii
1.4.3.2 N-ethylmaleimide 87% residual activity at 1 mM Penicillium steckii
1.4.3.2 NaN3 91% residual activity at 1 mM Penicillium steckii
1.4.3.2 Ni2+ 90% residual activity at 1 mM Penicillium steckii
1.4.3.2 o-phenanthroline 84% residual activity at 1 mM Penicillium steckii
1.4.3.2 Semicarbazide 70% residual activity at 1 mM Penicillium steckii
1.4.3.2 Zn2+ 55% residual activity at 1 mM Penicillium steckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.0061
-
 fish collagen peptide II at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.007
-
 Nepsilon-benzyloxycarbonyl-L-lysine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.0081
-
 fish collagen peptide I at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.01
-
 L-Lys at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.012
-
 salmon gelatin at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.017
-
 L-Orn at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.17
-
 beta-Lactoglobulin at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 0.25
-
 poly-L-lysine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 17
-
 Gly-L-Lys at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 26.5
-
 Nalpha-benzyloxycarbonyl-L-lysine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 37.7
-
 L-Ala-L-Lys at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii
1.4.3.2 56.4
-
 Nalpha-benzyloxycarbonyl-L-ornithine at pH 8.0 and 20°C, measured at the rate of H2O2 production Penicillium steckii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.2 Fe the enzyme contains iron Penicillium steckii
1.4.3.2 additional information not influenced by Cu2+ Penicillium steckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 290000
-
 gel filtration Penicillium steckii

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Penicillium steckii
-
-
-
1.4.3.2 Penicillium steckii AIU 027
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 phenyl Toyopearl column chromatography, DEAE Toyopearl column chromatography, aminooctyl Toyopearl column chromatography and hydroxyapatite column chromatography Penicillium steckii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 mycelium
-
 Penicillium steckii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 beta-lactoglobulin + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 fish collagen peptide I + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 fish collagen peptide II + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 Gly-L-Lys + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 L-Ala-L-Lys + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 L-Arg + H2O + O2
-
 Penicillium steckii 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2
-
 Penicillium steckii AIU 027 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2
-
 Penicillium steckii 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2
-
 Penicillium steckii AIU 027 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2
-
 Penicillium steckii 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Lys + H2O + O2
-
 Penicillium steckii 6-amino-2-oxo-hexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2
-
 Penicillium steckii 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2
-
 Penicillium steckii AIU 027 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Orn + H2O + O2
-
 Penicillium steckii 2-oxo-5-aminopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Orn + H2O + O2
-
 Penicillium steckii AIU 027 2-oxo-5-aminopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 additional information no activity with D-amino acids, aminoalcohols and amines as well as L-threonine, L-isoleucine, L-serine, L-valine, Lcysteine, L-aspartic acid, L-proline and glycine Penicillium steckii ?
-
 ?
1.4.3.2 additional information no activity with D-amino acids, aminoalcohols and amines as well as L-threonine, L-isoleucine, L-serine, L-valine, Lcysteine, L-aspartic acid, L-proline and glycine Penicillium steckii AIU 027 ?
-
 ?
1.4.3.2 myoglobin + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 Nalpha-benzyloxycarbonyl-L-lysine + H2O + O2
-
 Penicillium steckii Nalpha-benzyloxycarbonyl-L-aminoadipic delta-semialdehyde + NH3 + H2O2
-
 ?
1.4.3.2 Nalpha-benzyloxycarbonyl-L-ornithine + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 Nepsilon-benzyloxycarbonyl-L-lysine + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 poly-L-lysine + H2O + O2
-
 Penicillium steckii ?
-
 ?
1.4.3.2 salmon gelatin + H2O + O2
-
 Penicillium steckii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 homotetramer 4 * 75300, SDS-PAGE Penicillium steckii

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-AAO
-
 Penicillium steckii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 40
-
 when the enzyme is incubated at pH 6.0 for 30 min at various temperatures, the enzyme is stable blow 40°C Penicillium steckii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 5 7 the enzyme is stable for 30 min in the pH range from 5.0 to 6.0, but more than 90% of original activity is lost in the alkaline region from pH 7.0 Penicillium steckii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
 Penicillium steckii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Penicillium steckii isoelectric focusing
-
 3.2