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Literature summary extracted from

  • Huy, N.D.; Thayumanavan, P.; Kwon, T.H.; Park, S.M.
    Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium (2013), J. Biosci. Bioeng., 116, 152-159 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.72 Ca2+ 109% of initial activity Phanerodontia chrysosporium
3.1.1.72 Mg2+ 112% of initial activity Phanerodontia chrysosporium
3.2.1.37 D-glucose 5 mM, 123% of initial activity Phanerodontia chrysosporium
3.2.1.37 D-xylose 5 mM, 151% of initial activity Phanerodontia chrysosporium
3.2.1.37 L-arabinose 5 mM, 126% of initial activity Phanerodontia chrysosporium
3.2.1.55 D-glucose 5 mM, 123% of initial activity Phanerodontia chrysosporium
3.2.1.55 D-xylose 5 mM, 151% of initial activity Phanerodontia chrysosporium
3.2.1.55 L-arabinose 5 mM, 126% of initial activity Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 expression in Pichia pastoris Phanerodontia chrysosporium
3.2.1.37 exprerssion in Pichia pastoris Phanerodontia chrysosporium
3.2.1.55 exprerssion in Pichia pastoris Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.72 Co2+ 5 mM, less than 50% residual activity Phanerodontia chrysosporium
3.1.1.72 Cu2+ 5 mM, less than 50% residual activity Phanerodontia chrysosporium
3.1.1.72 Fe2+ 5 mM, 1.6% residual activity Phanerodontia chrysosporium
3.1.1.72 Mn2+ 5 mM, less than 50% residual activity Phanerodontia chrysosporium
3.1.1.72 Ni2+ 5 mM, less than 50% residual activity Phanerodontia chrysosporium
3.1.1.72 SDS 0.5%, 61% residual activity Phanerodontia chrysosporium
3.1.1.72 Triton X-100 1 mM, 78% residual activity Phanerodontia chrysosporium
3.2.1.37 Co2+ 10 mM, 20.4% residual activity Phanerodontia chrysosporium
3.2.1.37 Cu2+ 10 mM, 5.8% residual activity Phanerodontia chrysosporium
3.2.1.37 Fe2+ 10 mM, 47.6% residual activity Phanerodontia chrysosporium
3.2.1.37 Mn2+ 10 mM, 21.2% residual activity Phanerodontia chrysosporium
3.2.1.37 Ni2+ 10 mM, 17.2% residual activity Phanerodontia chrysosporium
3.2.1.37 Zn2+ 10 mM, 0.3% residual activity Phanerodontia chrysosporium
3.2.1.55 Co2+ 10 mM, 16.9% residual activity Phanerodontia chrysosporium
3.2.1.55 Cu2+ 10 mM, 2.3% residual activity Phanerodontia chrysosporium
3.2.1.55 Fe2+ 10 mM, 35.5% residual activity Phanerodontia chrysosporium
3.2.1.55 Mn2+ 10 mM, 23.8% residual activity Phanerodontia chrysosporium
3.2.1.55 Ni2+ 10 mM, 14.5% residual activity Phanerodontia chrysosporium
3.2.1.55 Zn2+ 10 mM, 0.1% residual activity Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 0.092
-
 4-nitrophenyl acetate pH 7.0, 30°C Phanerodontia chrysosporium
3.2.1.37 12.7
-
 4-nitrophenyl beta-D-xylopyranoside pH 5.0, 40°C Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Phanerodontia chrysosporium
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Phanerodontia chrysosporium H2ESB9
-
-
3.2.1.37 Phanerodontia chrysosporium K7T9D9 bifunctional beta-xylosidase/alpha-L-arabinofuranosidase, EC 3.2.1.37/EC 3.2.1.55
-
3.2.1.55 Phanerodontia chrysosporium K7T9D9 bifunctional beta-xylosidase/alpha-L-arabinofuranosidase, EC 3.2.1.37/EC 3.2.1.55
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.72 glycoprotein treatment with peptide-N-glycosidase and endoglycosidase H reduces the molecular mass to 55 kDa Phanerodontia chrysosporium
3.2.1.37 glycoprotein molecular mass of deglycosylated protein is 66000 Da Phanerodontia chrysosporium
3.2.1.55 glycoprotein molecular mass of deglycosylated protein is 66000 Da Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 39.86
-
 pH 7.0, 30°C Phanerodontia chrysosporium
3.2.1.37 1797
-
 substrate 4-nitrophenyl beta-D-xylopyranoside, pH 5.0, 40°C Phanerodontia chrysosporium
3.2.1.55 197
-
 substrate 4-nitrophenyl beta-D-xylopyranoside, pH 5.0, 40°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + acetate
-
 ?
3.1.1.72 additional information enzyme also exhibits a xylanase activity at an optimum pH and temperature of 5.0 and 80°C, respectively Phanerodontia chrysosporium ?
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 additional information alpha-L-arabinofuranosidase activity is about 10% of the beta-xylosidase activity. No substrates: 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-beta-D-glucopyranoside. Very poor substrates: birchwood xylan, beechwood xylan, sugar beet arabinan, debranched arabinana, wheat arabinoxylan Phanerodontia chrysosporium ?
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 additional information alpha-L-arabinofuranosidase activity is about 10% of the beta-xylosidase activity. No substrates: 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-beta-D-glucopyranoside. Very poor substrates: birchwood xylan, beechwood xylan, sugar beet arabinan, debranched arabinana, wheat arabinoxylan Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 63000, SDS-PAGE, recombinant hyperglycosylated protein, x * 37000, calculated Phanerodontia chrysosporium
3.2.1.37 ? x * 83000, SDS-PAGE, x * 65600, calculated Phanerodontia chrysosporium
3.2.1.55 ? x * 83000, SDS-PAGE, x * 65600, calculated Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 Axe2
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 30 35
-
 Phanerodontia chrysosporium
3.2.1.37 45
-
-
 Phanerodontia chrysosporium
3.2.1.55 45
-
-
 Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 7
-
-
 Phanerodontia chrysosporium
3.2.1.37 5
-
-
 Phanerodontia chrysosporium
3.2.1.55 5
-
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.72 6.5 7.5
-
 Phanerodontia chrysosporium

General Information

EC Number General Information Comment Organism
3.1.1.72 physiological function the synergistic action of Axe2 with endo-xylanase XynC on birchwood xylan, beechwood xylan and wheat arabinoxylan enhances the total reducing soluble sugar Phanerodontia chrysosporium