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Literature summary extracted from

  • Kung, J.W.; Meier, A.K.; Mergelsberg, M.; Boll, M.
    Enzymes involved in a novel anaerobic cyclohexane carboxylic acid degradation pathway (2014), J. Bacteriol., 196, 3667-3674 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.10 0.031
-
 cyclohex-1-ene-1-carbonyl-CoA pH 6.8, 30°C Geobacter metallireducens
1.3.8.10 0.085
-
 cyclohex-1,5-diene-1-carbonyl-CoA pH 6.8, 30°C Geobacter metallireducens
1.3.8.11 0.005
-
 cyclohexane-1-carbonyl-CoA pH 6.8, 30°C Geobacter metallireducens

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.10 Geobacter metallireducens Q39QF5
-
-
1.3.8.10 Geobacter metallireducens DSM 7210 Q39QF5
-
-
1.3.8.11 Geobacter metallireducens Q39QF4
-
-
1.3.8.11 Geobacter metallireducens DSM 7210 Q39QF4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.10 1
-
 substrate cyclohex-1,5-diene-1-carbonyl-CoA, pH 6.8, 30°C Geobacter metallireducens
1.3.8.10 2.3
-
 substrate cyclohex-1-ene-1-carbonyl-CoA, pH 6.8, 30°C Geobacter metallireducens
1.3.8.11 29
-
 pH 6.8, 30°C Geobacter metallireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.10 cyclohex-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens benzoyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.10 cyclohex-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens DSM 7210 benzoyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.10 cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.10 cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens DSM 7210 cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.10 additional information no substrates: cyclohexanoyl-CoA, cyclohex-2-ene-1-carboxyl-CoA, cyclohex-3-ene-1-carboxyl-CoA, butanoyl-CoA, nor crotonyl-CoA Geobacter metallireducens ?
-
 ?
1.3.8.10 additional information no substrates: cyclohexanoyl-CoA, cyclohex-2-ene-1-carboxyl-CoA, cyclohex-3-ene-1-carboxyl-CoA, butanoyl-CoA, nor crotonyl-CoA Geobacter metallireducens DSM 7210 ?
-
 ?
1.3.8.11 cyclohex-3-ene-1-carboxyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens cyclohexa-1,3-diene-1-carboxyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.11 cyclohex-3-ene-1-carboxyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens DSM 7210 cyclohexa-1,3-diene-1-carboxyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.11 cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.11 cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
 Geobacter metallireducens DSM 7210 cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
 ?
1.3.8.11 additional information product is not further dehydrogenated to form cyclohex-1,5-diene-1-carbonyl-CoA. No substrates: 6-hydroxycyclohex-1-ene-1-carboxyl-CoA, cyclohex-2-ene-1-carboxyl-CoA, butanoyl-CoA, crotonyl-CoA, isobutyryl-CoA, glutaryl-CoA, or succinyl-CoA Geobacter metallireducens ?
-
 ?
1.3.8.11 additional information product is not further dehydrogenated to form cyclohex-1,5-diene-1-carbonyl-CoA. No substrates: 6-hydroxycyclohex-1-ene-1-carboxyl-CoA, cyclohex-2-ene-1-carboxyl-CoA, butanoyl-CoA, crotonyl-CoA, isobutyryl-CoA, glutaryl-CoA, or succinyl-CoA Geobacter metallireducens DSM 7210 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.10 ? x * 40900,SDS-PAGE, recombinant protein with His-tag Geobacter metallireducens
1.3.8.11 ? x * 41200,SDS-PAGE, recombinant protein with His-tag Geobacter metallireducens

Synonyms

EC Number Synonyms Comment Organism
1.3.8.10 Gmet_3306
-
 Geobacter metallireducens
1.3.8.11 Gmet_3307
-
 Geobacter metallireducens

General Information

EC Number General Information Comment Organism
1.3.8.10 physiological function Geobacter metallireducens grows exponentially with cyclohexane carboxylic acid as the sole carbon source and nitrate as a terminal electron acceptor. Cyclohexane carboxylic acid degradation involves its activation by a succinyl-CoA-dependent CoA transferase followed by the oxidation to cyclohexadienoyl-CoA by cyclohexanoyl-CoA- and cyclohex-1-ene-1-carboxyl-CoA dehydrogenase activities Geobacter metallireducens
1.3.8.11 physiological function Geobacter metallireducens grows exponentially with cyclohexane carboxylic acid as the sole carbon source and nitrate as a terminal electron acceptor. Cyclohexane carboxylic acid degradation involves its activation by a succinyl-CoA-dependent CoA transferase followed by the oxidation to cyclohexadienoyl-CoA by cyclohexanoyl-CoA- and cyclohex-1-ene-1-carboxyl-CoA dehydrogenase activities Geobacter metallireducens