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Literature summary extracted from

  • Lockwood, C.W.; Burlat, B.; Cheesman, M.R.; Kern, M.; Simon, J.; Clarke, T.A.; Richardson, D.J.; Butt, J.N.
    Resolution of key roles for the distal pocket histidine in cytochrome C nitrite reductases (2015), J. Am. Chem. Soc., 137, 3059-3068 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.2 expression in Escherichia coli Escherichia coli
1.7.2.2 expression in Escherichia coli Wolinella succinogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.2 mutant H264N, to 2.15 A resolution. Homodimeric protein, the constellation of His/His and Lys-coordinated c-hemes is indistinguishable from those of the native enzyme Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.2 H264N mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme Escherichia coli
1.7.2.2 H277N mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme Wolinella succinogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.2 0.046
-
 nitrite wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.7.2.2 0.08
-
 nitrite wild-type, pH 7.0, temperature not specified in the publication Wolinella succinogenes
1.7.2.2 2.01
-
 hydroxylamine wild-type, pH 7.0, temperature not specified in the publication Wolinella succinogenes
1.7.2.2 2.03
-
 hydroxylamine mutant H264N, pH 7.0, temperature not specified in the publication Wolinella succinogenes
1.7.2.2 30
-
 hydroxylamine wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.7.2.2 170
-
 hydroxylamine mutant H264N, pH 7.0, temperature not specified in the publication Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Escherichia coli P0ABK9
-
-
1.7.2.2 Wolinella succinogenes Q9S1E5
-
-
1.7.2.2 Wolinella succinogenes DSM 1740 Q9S1E5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 hydroxylamine + reduced benzyl viologen
-
 Escherichia coli ? + oxidized benzyl viologen
-
 ?
1.7.2.2 hydroxylamine + reduced benzyl viologen
-
 Wolinella succinogenes ? + oxidized benzyl viologen
-
 ?
1.7.2.2 hydroxylamine + reduced benzyl viologen
-
 Wolinella succinogenes DSM 1740 ? + oxidized benzyl viologen
-
 ?
1.7.2.2 nitrite + reduced methyl viologen
-
 Escherichia coli NH3 + H2O + oxidized methyl viologen
-
 ?
1.7.2.2 nitrite + reduced methyl viologen
-
 Wolinella succinogenes NH3 + H2O + oxidized methyl viologen
-
 ?
1.7.2.2 nitrite + reduced methyl viologen
-
 Wolinella succinogenes DSM 1740 NH3 + H2O + oxidized methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 cytochrome c-552
-
 Escherichia coli
1.7.2.2 cytochrome c-552
-
 Wolinella succinogenes
1.7.2.2 NrfA
-
 Escherichia coli
1.7.2.2 NrfA
-
 Wolinella succinogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.2 197
-
 hydroxylamine mutant H264N, pH 7.0, temperature not specified in the publication Wolinella succinogenes
1.7.2.2 215
-
 hydroxylamine wild-type, pH 7.0, temperature not specified in the publication Wolinella succinogenes
1.7.2.2 758
-
 nitrite wild-type, pH 7.0, temperature not specified in the publication Wolinella succinogenes
1.7.2.2 1350
-
 nitrite wild-type, pH 7.0, temperature not specified in the publication Escherichia coli
1.7.2.2 3100
-
 hydroxylamine mutant H264N, pH 7.0, temperature not specified in the publication Escherichia coli
1.7.2.2 3160
-
 hydroxylamine wild-type, pH 7.0, temperature not specified in the publication Escherichia coli