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Literature summary extracted from

  • Thakur, N.; Gupta, S.; Hade, M.D.; Dikshit, K.L.
    Type I flavohemoglobin of Mycobacterium smegmatis is a functional nitric oxide dioxygenase (2014), IUBMB Life, 66, 396-404 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.17 expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.17 nitric oxide + O2 + NADH Mycolicibacterium smegmatis
-
 nitrate + NAD+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NADH Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 nitrate + NAD+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NADPH Mycolicibacterium smegmatis
-
 nitrate + NADP+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NADPH Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 nitrate + NADP+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.17 Mycolicibacterium smegmatis
-
-
-
1.14.12.17 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.17 metal affinity chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.17 nitric oxide + O2 + NADH
-
 Mycolicibacterium smegmatis nitrate + NAD+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NADH
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 nitrate + NAD+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NADPH
-
 Mycolicibacterium smegmatis nitrate + NADP+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NADPH
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 nitrate + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.17 ? x * 44000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
1.14.12.17 FHb1
-
 Mycolicibacterium smegmatis
1.14.12.17 NO dioxygenase
-
 Mycolicibacterium smegmatis
1.14.12.17 NOD
-
 Mycolicibacterium smegmatis
1.14.12.17 type I flavohemoglobin
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.17 FAD
-
 Mycolicibacterium smegmatis
1.14.12.17 heme
-
 Mycolicibacterium smegmatis
1.14.12.17 NADH the enzyme shows preference for NADH over NADPH Mycolicibacterium smegmatis
1.14.12.17 NADPH the enzyme shows preference for NADH over NADPH Mycolicibacterium smegmatis

Expression

EC Number Organism Comment Expression
1.14.12.17 Mycolicibacterium smegmatis the level of enzyme transcript increases significantly in the presence of acidified nitrite up