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Literature summary extracted from

  • Park, N.; Park, S.S.
    Purification and characterization of a novel laccase from Fomitopsis pinicola mycelia (2014), Int. J. Biol. Macromol., 70, 583-589 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatmen Fomitopsis pinicola
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Fomitopsis pinicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Al3+ 5 mM, 44% inhibition Fomitopsis pinicola
1.10.3.2 Ba2+ 5 mM, 27% inhibition Fomitopsis pinicola
1.10.3.2 Ca2+ 5 mM, 17% inhibition Fomitopsis pinicola
1.10.3.2 Co2+ 1 mM, complete inhibition Fomitopsis pinicola
1.10.3.2 Cu2+ 10 mM, 25% inhibition Fomitopsis pinicola
1.10.3.2 Mg2+ 5 mM, 34% inhibition Fomitopsis pinicola
1.10.3.2 Mn2+ 5 mM, 20% inhibition Fomitopsis pinicola
1.10.3.2 NaN3
-
 Fomitopsis pinicola
1.10.3.2 Ni2+ 5 mM, 25% inhibition Fomitopsis pinicola
1.10.3.2 SDS
-
 Fomitopsis pinicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.28
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Al3+ 1 mM, 1.1fold activation Fomitopsis pinicola
1.10.3.2 Ba2+ 1 mM, 1.15fold activation Fomitopsis pinicola
1.10.3.2 Ca2+ 1 mM, 1.24fold activation Fomitopsis pinicola
1.10.3.2 Cu2+ 1 mM, 1.38fold activation Fomitopsis pinicola
1.10.3.2 K+ 1 mM, 1.3fold activation Fomitopsis pinicola
1.10.3.2 Mg2+ 1 mM, 1.16fold activation Fomitopsis pinicola
1.10.3.2 Mn2+ 1 mM, 1.3fold activation Fomitopsis pinicola
1.10.3.2 Ni2+ 1 mM, 1.3fold activation Fomitopsis pinicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 92000
-
 gel filtration Fomitopsis pinicola

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Fomitopsis pinicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Fomitopsis pinicola

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Fomitopsis pinicola
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Fomitopsis pinicola ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 7.1% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Fomitopsis pinicola ?
-
 ?
1.10.3.2 guaiacol + O2 1.4% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Fomitopsis pinicola ?
-
 ?
1.10.3.2 o-tolidine + O2 9.0% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Fomitopsis pinicola ?
-
 ?
1.10.3.2 syringaldazine + O2 0.5% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Fomitopsis pinicola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 92000, SDS-PAGE Fomitopsis pinicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 80
-
-
 Fomitopsis pinicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 stable below Fomitopsis pinicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
-
 Fomitopsis pinicola

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 1.5 11 enzyme is relatively stable Fomitopsis pinicola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Fomitopsis pinicola
-
-
 3.8