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Literature summary extracted from

  • Kaur, P.; Patil, H.; Bhanushali, P.; Badgujar, S.; Gupta, A.
    Purification of recombinant human thyroid peroxidase (hTPO) from AD293 mammalian cells (2018), Int. J. Biol. Macromol., 106, 87-94 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.8 synthesis expression and purification of recombinant protein from AD-293 cells unsing the aminoterminal cDNA sequence of luciferase protein from Gaussia princeps for secretion. A simplified purification procedure consists of a couple of anionexchange chromatography columns Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.8 expression in AD-293 cell Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.8 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.8 180
-
 pH 7.4, 25°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.11.1.8 -70°C, purified recombinant protein treated with 600 microM hemin, stable for for 4 years Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.8 guaiacol + H2O2
-
 Homo sapiens 3,3'-dimethoxy-4,4'-biphenoquinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.8 ? x * 105000, SDS-PAGE of recombinant protein Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.8 heme heme prosthetic group of TPO is stable and gives a A412/A280ratio of 0.38 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.8 Homo sapiens calculated
-
 6.1