BRENDA - Enzyme Database

Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis

Wakamatsu, T.; Higashi, C.; Ohmori, T.; Doi, K.; Ohshima, T.; Extremophiles 17, 379-389 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.4.1.2
isocitrate
10 M, 112% of initial activity
Pyrobaculum calidifontis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.2
malate
10 mM, 75% residual activity
Pyrobaculum calidifontis
1.4.1.2
oxaloacetate
10 mM, 60% residual activity
Pyrobaculum calidifontis
1.4.1.4
Hg2+
1 mM, 76% residual activity
Pyrobaculum calidifontis
1.4.1.4
isocitrate
10 M, 64% of initial activity
Pyrobaculum calidifontis
1.4.1.4
KCl
100 mM, 45% residual activity
Pyrobaculum calidifontis
1.4.1.4
NaCl
100 mM, 59% residual activity
Pyrobaculum calidifontis
1.4.1.4
oxaloacetate
10 mM, 63% residual activity
Pyrobaculum calidifontis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.2
0.01
-
NADH
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
0.028
-
NAD+
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
0.92
-
2-oxoglutarate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
5.3
-
L-glutamate
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
9.8
-
NH3
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
0.017
-
NADPH
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
0.035
-
NADP+
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
1.7
-
2-oxoglutarate
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
2.2
-
NH3
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
3.4
-
L-glutamate
pH 9.5, 50°C
Pyrobaculum calidifontis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.1.2
Mn2+
1 mM, 109% of initial activity
Pyrobaculum calidifontis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.2
286000
-
gel filtration
Pyrobaculum calidifontis
1.4.1.4
270000
-
gel filtration
Pyrobaculum calidifontis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.1.2
Pyrobaculum calidifontis
A3MUY9
-
-
1.4.1.2
Pyrobaculum calidifontis JCM 11548
A3MUY9
-
-
1.4.1.4
Pyrobaculum calidifontis
A3MWK6
-
-
1.4.1.4
Pyrobaculum calidifontis JCM 11548
A3MWK6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.2
2-oxobutanoate + NH3 + NADH + H+
2.7% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-2-aminobutanoate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxobutanoate + NH3 + NADH + H+
2.7% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis JCM 11548
L-2-aminobutanoate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxocaproate + NH3 + NADH + H+
1.3% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-2-aminohexanoate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxoglutarate + NH3 + NADH + H+
-
742464
Pyrobaculum calidifontis
L-glutamate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxoglutarate + NH3 + NADH + H+
-
742464
Pyrobaculum calidifontis JCM 11548
L-glutamate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxovalerate + NH3 + NADH + H+
6.6% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-valine + H2O + NAD+
-
-
-
r
1.4.1.2
L-2-aminobutanoate + H2O + NAD+
8.4% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-2-aminobutanoate + H2O + NAD+
8.4% of the activity with L-glutamate
742464
Pyrobaculum calidifontis JCM 11548
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-glutamate + H2O + NAD+
-
742464
Pyrobaculum calidifontis
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-glutamate + H2O + NAD+
-
742464
Pyrobaculum calidifontis JCM 11548
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-isoleucine + H2O + NAD+
3.0% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxo-3-methylvalerate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-leucine + H2O + NAD+
1.6% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxo-iso-valerate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-norvaline + H2O + NAD+
35% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-norvaline + H2O + NAD+
35% of the activity with L-glutamate
742464
Pyrobaculum calidifontis JCM 11548
2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-valine + H2O + NAD+
31% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxovalerate + NH3 + NADH + H+
-
-
-
r
1.4.1.4
2-oxobutanoate + NH3 + NADPH + H+
2.6% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-2-aminobutanoate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxobutanoate + NH3 + NADPH + H+
2.6% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis JCM 11548
L-2-aminobutanoate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxoglutarate + NH3 + NADPH + H+
-
742464
Pyrobaculum calidifontis
L-glutamate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxoglutarate + NH3 + NADPH + H+
-
742464
Pyrobaculum calidifontis JCM 11548
L-glutamate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxovalerate + NH3 + NADPH + H+
4.2% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-valine + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxovalerate + NH3 + NADPH + H+
4.2% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis JCM 11548
L-valine + H2O + NADP+
-
-
-
r
1.4.1.4
L-glutamate + H2O + NADP+
-
742464
Pyrobaculum calidifontis
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
1.4.1.4
L-glutamate + H2O + NADP+
-
742464
Pyrobaculum calidifontis JCM 11548
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
1.4.1.4
L-norvaline + H2O + NADP+
3% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxopentanoate + NH3 + NADPH + H+
-
-
-
r
1.4.1.4
L-norvaline + H2O + NADP+
3% of the activity with L-glutamate
742464
Pyrobaculum calidifontis JCM 11548
2-oxopentanoate + NH3 + NADPH + H+
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.4.1.2
hexamer
6 * 48200, calculated
Pyrobaculum calidifontis
1.4.1.4
hexamer
6 * 47700, calculated
Pyrobaculum calidifontis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.1.2
14
-
NAD+
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
17
-
L-glutamate
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
58
-
NADH
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
58
-
NH3
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
65
-
2-oxoglutarate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
9.9
-
NADP+
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
13
-
L-glutamate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
92
-
NADPH
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
95
-
2-oxoglutarate
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
100
-
NH3
pH 9.0, 50°C
Pyrobaculum calidifontis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.2
additional information
no cofactor: NADP+
Pyrobaculum calidifontis
1.4.1.2
NAD+
-
Pyrobaculum calidifontis
1.4.1.2
NADH
-
Pyrobaculum calidifontis
1.4.1.2
NADPH
about 1% of th activity with NADH
Pyrobaculum calidifontis
1.4.1.4
NAD+
about 17% of the activity with NADP+
Pyrobaculum calidifontis
1.4.1.4
NADH
about 7% of the activity with NADPH
Pyrobaculum calidifontis
1.4.1.4
NADP+
-
Pyrobaculum calidifontis
1.4.1.4
NADPH
-
Pyrobaculum calidifontis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.4.1.2
isocitrate
10 M, 112% of initial activity
Pyrobaculum calidifontis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.2
additional information
no cofactor: NADP+
Pyrobaculum calidifontis
1.4.1.2
NAD+
-
Pyrobaculum calidifontis
1.4.1.2
NADH
-
Pyrobaculum calidifontis
1.4.1.2
NADPH
about 1% of th activity with NADH
Pyrobaculum calidifontis
1.4.1.4
NAD+
about 17% of the activity with NADP+
Pyrobaculum calidifontis
1.4.1.4
NADH
about 7% of the activity with NADPH
Pyrobaculum calidifontis
1.4.1.4
NADP+
-
Pyrobaculum calidifontis
1.4.1.4
NADPH
-
Pyrobaculum calidifontis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.2
malate
10 mM, 75% residual activity
Pyrobaculum calidifontis
1.4.1.2
oxaloacetate
10 mM, 60% residual activity
Pyrobaculum calidifontis
1.4.1.4
Hg2+
1 mM, 76% residual activity
Pyrobaculum calidifontis
1.4.1.4
isocitrate
10 M, 64% of initial activity
Pyrobaculum calidifontis
1.4.1.4
KCl
100 mM, 45% residual activity
Pyrobaculum calidifontis
1.4.1.4
NaCl
100 mM, 59% residual activity
Pyrobaculum calidifontis
1.4.1.4
oxaloacetate
10 mM, 63% residual activity
Pyrobaculum calidifontis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.2
0.01
-
NADH
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
0.028
-
NAD+
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
0.92
-
2-oxoglutarate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
5.3
-
L-glutamate
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
9.8
-
NH3
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
0.017
-
NADPH
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
0.035
-
NADP+
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
1.7
-
2-oxoglutarate
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
2.2
-
NH3
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
3.4
-
L-glutamate
pH 9.5, 50°C
Pyrobaculum calidifontis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.1.2
Mn2+
1 mM, 109% of initial activity
Pyrobaculum calidifontis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.2
286000
-
gel filtration
Pyrobaculum calidifontis
1.4.1.4
270000
-
gel filtration
Pyrobaculum calidifontis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.2
2-oxobutanoate + NH3 + NADH + H+
2.7% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-2-aminobutanoate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxobutanoate + NH3 + NADH + H+
2.7% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis JCM 11548
L-2-aminobutanoate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxocaproate + NH3 + NADH + H+
1.3% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-2-aminohexanoate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxoglutarate + NH3 + NADH + H+
-
742464
Pyrobaculum calidifontis
L-glutamate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxoglutarate + NH3 + NADH + H+
-
742464
Pyrobaculum calidifontis JCM 11548
L-glutamate + H2O + NAD+
-
-
-
r
1.4.1.2
2-oxovalerate + NH3 + NADH + H+
6.6% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-valine + H2O + NAD+
-
-
-
r
1.4.1.2
L-2-aminobutanoate + H2O + NAD+
8.4% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-2-aminobutanoate + H2O + NAD+
8.4% of the activity with L-glutamate
742464
Pyrobaculum calidifontis JCM 11548
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-glutamate + H2O + NAD+
-
742464
Pyrobaculum calidifontis
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-glutamate + H2O + NAD+
-
742464
Pyrobaculum calidifontis JCM 11548
2-oxoglutarate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-isoleucine + H2O + NAD+
3.0% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxo-3-methylvalerate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-leucine + H2O + NAD+
1.6% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxo-iso-valerate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-norvaline + H2O + NAD+
35% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-norvaline + H2O + NAD+
35% of the activity with L-glutamate
742464
Pyrobaculum calidifontis JCM 11548
2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
1.4.1.2
L-valine + H2O + NAD+
31% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxovalerate + NH3 + NADH + H+
-
-
-
r
1.4.1.4
2-oxobutanoate + NH3 + NADPH + H+
2.6% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-2-aminobutanoate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxobutanoate + NH3 + NADPH + H+
2.6% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis JCM 11548
L-2-aminobutanoate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxoglutarate + NH3 + NADPH + H+
-
742464
Pyrobaculum calidifontis
L-glutamate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxoglutarate + NH3 + NADPH + H+
-
742464
Pyrobaculum calidifontis JCM 11548
L-glutamate + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxovalerate + NH3 + NADPH + H+
4.2% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis
L-valine + H2O + NADP+
-
-
-
r
1.4.1.4
2-oxovalerate + NH3 + NADPH + H+
4.2% of the activity with 2-oxoglutarate
742464
Pyrobaculum calidifontis JCM 11548
L-valine + H2O + NADP+
-
-
-
r
1.4.1.4
L-glutamate + H2O + NADP+
-
742464
Pyrobaculum calidifontis
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
1.4.1.4
L-glutamate + H2O + NADP+
-
742464
Pyrobaculum calidifontis JCM 11548
2-oxoglutarate + NH3 + NADPH + H+
-
-
-
r
1.4.1.4
L-norvaline + H2O + NADP+
3% of the activity with L-glutamate
742464
Pyrobaculum calidifontis
2-oxopentanoate + NH3 + NADPH + H+
-
-
-
r
1.4.1.4
L-norvaline + H2O + NADP+
3% of the activity with L-glutamate
742464
Pyrobaculum calidifontis JCM 11548
2-oxopentanoate + NH3 + NADPH + H+
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.1.2
hexamer
6 * 48200, calculated
Pyrobaculum calidifontis
1.4.1.4
hexamer
6 * 47700, calculated
Pyrobaculum calidifontis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.1.2
14
-
NAD+
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
17
-
L-glutamate
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
58
-
NADH
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
58
-
NH3
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
65
-
2-oxoglutarate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
9.9
-
NADP+
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
13
-
L-glutamate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
92
-
NADPH
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
95
-
2-oxoglutarate
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
100
-
NH3
pH 9.0, 50°C
Pyrobaculum calidifontis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4.1.2
3.2
-
L-glutamate
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
5.9
-
NH3
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
71
-
2-oxoglutarate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
500
-
NAD+
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
5800
-
NADH
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
3.8
-
L-glutamate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
45
-
NH3
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
56
-
2-oxoglutarate
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
280
-
NADP+
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
5400
-
NADPH
pH 9.0, 50°C
Pyrobaculum calidifontis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.4.1.2
3.2
-
L-glutamate
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
5.9
-
NH3
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
71
-
2-oxoglutarate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
500
-
NAD+
pH 10.5, 50°C
Pyrobaculum calidifontis
1.4.1.2
5800
-
NADH
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
3.8
-
L-glutamate
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
45
-
NH3
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
56
-
2-oxoglutarate
pH 9.0, 50°C
Pyrobaculum calidifontis
1.4.1.4
280
-
NADP+
pH 9.5, 50°C
Pyrobaculum calidifontis
1.4.1.4
5400
-
NADPH
pH 9.0, 50°C
Pyrobaculum calidifontis