EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | isocitrate | 10 M, 112% of initial activity | Pyrobaculum calidifontis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | malate | 10 mM, 75% residual activity | Pyrobaculum calidifontis | |
1.4.1.2 | oxaloacetate | 10 mM, 60% residual activity | Pyrobaculum calidifontis | |
1.4.1.4 | Hg2+ | 1 mM, 76% residual activity | Pyrobaculum calidifontis | |
1.4.1.4 | isocitrate | 10 M, 64% of initial activity | Pyrobaculum calidifontis | |
1.4.1.4 | KCl | 100 mM, 45% residual activity | Pyrobaculum calidifontis | |
1.4.1.4 | NaCl | 100 mM, 59% residual activity | Pyrobaculum calidifontis | |
1.4.1.4 | oxaloacetate | 10 mM, 63% residual activity | Pyrobaculum calidifontis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.2 | 0.01 | - |
NADH | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 0.028 | - |
NAD+ | pH 10.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 0.92 | - |
2-oxoglutarate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 5.3 | - |
L-glutamate | pH 10.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 9.8 | - |
NH3 | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 0.017 | - |
NADPH | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 0.035 | - |
NADP+ | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 1.7 | - |
2-oxoglutarate | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 2.2 | - |
NH3 | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 3.4 | - |
L-glutamate | pH 9.5, 50°C | Pyrobaculum calidifontis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | Mn2+ | 1 mM, 109% of initial activity | Pyrobaculum calidifontis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.2 | 286000 | - |
gel filtration | Pyrobaculum calidifontis |
1.4.1.4 | 270000 | - |
gel filtration | Pyrobaculum calidifontis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.2 | Pyrobaculum calidifontis | A3MUY9 | - |
- |
1.4.1.2 | Pyrobaculum calidifontis JCM 11548 | A3MUY9 | - |
- |
1.4.1.4 | Pyrobaculum calidifontis | A3MWK6 | - |
- |
1.4.1.4 | Pyrobaculum calidifontis JCM 11548 | A3MWK6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.2 | 2-oxobutanoate + NH3 + NADH + H+ | 2.7% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
1.4.1.2 | 2-oxobutanoate + NH3 + NADH + H+ | 2.7% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis JCM 11548 | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
1.4.1.2 | 2-oxocaproate + NH3 + NADH + H+ | 1.3% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-2-aminohexanoate + H2O + NAD+ | - |
r | |
1.4.1.2 | 2-oxoglutarate + NH3 + NADH + H+ | - |
Pyrobaculum calidifontis | L-glutamate + H2O + NAD+ | - |
r | |
1.4.1.2 | 2-oxoglutarate + NH3 + NADH + H+ | - |
Pyrobaculum calidifontis JCM 11548 | L-glutamate + H2O + NAD+ | - |
r | |
1.4.1.2 | 2-oxovalerate + NH3 + NADH + H+ | 6.6% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-valine + H2O + NAD+ | - |
r | |
1.4.1.2 | L-2-aminobutanoate + H2O + NAD+ | 8.4% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-2-aminobutanoate + H2O + NAD+ | 8.4% of the activity with L-glutamate | Pyrobaculum calidifontis JCM 11548 | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-glutamate + H2O + NAD+ | - |
Pyrobaculum calidifontis | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-glutamate + H2O + NAD+ | - |
Pyrobaculum calidifontis JCM 11548 | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-isoleucine + H2O + NAD+ | 3.0% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxo-3-methylvalerate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-leucine + H2O + NAD+ | 1.6% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxo-iso-valerate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-norvaline + H2O + NAD+ | 35% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-norvaline + H2O + NAD+ | 35% of the activity with L-glutamate | Pyrobaculum calidifontis JCM 11548 | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-valine + H2O + NAD+ | 31% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
1.4.1.4 | 2-oxobutanoate + NH3 + NADPH + H+ | 2.6% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-2-aminobutanoate + H2O + NADP+ | - |
r | |
1.4.1.4 | 2-oxobutanoate + NH3 + NADPH + H+ | 2.6% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis JCM 11548 | L-2-aminobutanoate + H2O + NADP+ | - |
r | |
1.4.1.4 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
Pyrobaculum calidifontis | L-glutamate + H2O + NADP+ | - |
r | |
1.4.1.4 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
Pyrobaculum calidifontis JCM 11548 | L-glutamate + H2O + NADP+ | - |
r | |
1.4.1.4 | 2-oxovalerate + NH3 + NADPH + H+ | 4.2% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-valine + H2O + NADP+ | - |
r | |
1.4.1.4 | 2-oxovalerate + NH3 + NADPH + H+ | 4.2% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis JCM 11548 | L-valine + H2O + NADP+ | - |
r | |
1.4.1.4 | L-glutamate + H2O + NADP+ | - |
Pyrobaculum calidifontis | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
1.4.1.4 | L-glutamate + H2O + NADP+ | - |
Pyrobaculum calidifontis JCM 11548 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
1.4.1.4 | L-norvaline + H2O + NADP+ | 3% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxopentanoate + NH3 + NADPH + H+ | - |
r | |
1.4.1.4 | L-norvaline + H2O + NADP+ | 3% of the activity with L-glutamate | Pyrobaculum calidifontis JCM 11548 | 2-oxopentanoate + NH3 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.2 | hexamer | 6 * 48200, calculated | Pyrobaculum calidifontis |
1.4.1.4 | hexamer | 6 * 47700, calculated | Pyrobaculum calidifontis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.2 | Pcal_1031 | - |
Pyrobaculum calidifontis |
1.4.1.4 | Pcal_1606 | - |
Pyrobaculum calidifontis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.2 | 14 | - |
NAD+ | pH 10.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 17 | - |
L-glutamate | pH 10.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 58 | - |
NADH | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 58 | - |
NH3 | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 65 | - |
2-oxoglutarate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 9.9 | - |
NADP+ | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 13 | - |
L-glutamate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 92 | - |
NADPH | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 95 | - |
2-oxoglutarate | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 100 | - |
NH3 | pH 9.0, 50°C | Pyrobaculum calidifontis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | additional information | no cofactor: NADP+ | Pyrobaculum calidifontis | |
1.4.1.2 | NAD+ | - |
Pyrobaculum calidifontis | |
1.4.1.2 | NADH | - |
Pyrobaculum calidifontis | |
1.4.1.2 | NADPH | about 1% of th activity with NADH | Pyrobaculum calidifontis | |
1.4.1.4 | NAD+ | about 17% of the activity with NADP+ | Pyrobaculum calidifontis | |
1.4.1.4 | NADH | about 7% of the activity with NADPH | Pyrobaculum calidifontis | |
1.4.1.4 | NADP+ | - |
Pyrobaculum calidifontis | |
1.4.1.4 | NADPH | - |
Pyrobaculum calidifontis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.2 | 3.2 | - |
L-glutamate | pH 10.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 5.9 | - |
NH3 | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 71 | - |
2-oxoglutarate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 500 | - |
NAD+ | pH 10.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.2 | 5800 | - |
NADH | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 3.8 | - |
L-glutamate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 45 | - |
NH3 | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 56 | - |
2-oxoglutarate | pH 9.0, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 280 | - |
NADP+ | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.4.1.4 | 5400 | - |
NADPH | pH 9.0, 50°C | Pyrobaculum calidifontis |