Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Morales-Alvarez, E.D.; Rivera-Hoyos, C.M.; Cardozo-Bernal, A.M.; Poutou-Pinales, R.A.; Pedroza-Rodriguez, A.M.; Diaz-Rincon, D.J.; Rodriguez-Lopez, A.; Almeciga-Diaz, C.J.; Cuervo-Patino, C.L.
    Plackett-Burman Design for rGILCC1 laccase activity enhancement in Pichia pastoris concentrated enzyme kinetic characterization (2017), Enzyme Res., 2017, 5947581 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression in Pichia pastoris Ganoderma lucidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0536
-
 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 25°C, pH 4.5 Ganoderma lucidum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Ganoderma lucidum V9PQZ7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Ganoderma lucidum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 23
-
 assay at Ganoderma lucidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 1 h, stable up to Ganoderma lucidum
1.10.3.2 60
-
 1 h, 10% loss of activity Ganoderma lucidum
1.10.3.2 70
-
 1 h, 40% loss of activity Ganoderma lucidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.5
-
 assay at Ganoderma lucidum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 11 for pH ranges between pH 2 and pH 11 enzyme activity ranges between 75 and 100% Ganoderma lucidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2 11 1 h, stable Ganoderma lucidum