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Literature summary extracted from

  • Zhu, Q.; El-Mergawy, R.G.; Zhou, Y.; Chen, C.; Heinemann, S.H.; Schoenherr, R.; Robaa, D.; Sippl, W.; Scriba, G.K.
    Stereospecific capillary electrophoresis assays using pentapeptide substrates for the study of Aspergillus nidulans methionine sulfoxide reductase A and mutant enzymes (2016), Electrophoresis, 37, 2083-2090 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.11 molecular modeling. The conserved residue Glu99 is buried in the Met-S-(O) groove, which might contribute to the correct placing of substrates. Residue Asp134 does not form hydrogen bonds with the substrates but only within the enzyme Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 D134E most active among the mutant enzymes tested, less active than wild-type Aspergillus nidulans
1.8.4.11 D134N lowest activity among all mutants tested for ac-L-Lys-L-Asn-l-Met(O)-L-Asp-L-Lys-dinitrophenol Aspergillus nidulans
1.8.4.11 E99Q lowest activity towards all substrates tested except for ac-L-Lys-L-Asn-l-Met(O)-L-Asp-L-Lys-dinitrophenol Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Aspergillus nidulans
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 ac-L-Lys-L-Asn-L-Met(O)-L-Asp-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Asn-L-Met-L-Asp-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?
1.8.4.11 ac-L-Lys-L-Asp-L-Met(O)-L-Asn-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Asp-L-Met-L-Asn-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?
1.8.4.11 ac-L-Lys-L-Asp-L-Met(O)-L-Asp-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Asp-L-Met-L-Asp-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?
1.8.4.11 ac-L-Lys-L-Phe-L-Met(O)-L-Lys-L-Lys-dinitrophenol + dithiothreitol
-
 Aspergillus nidulans ac-L-Lys-L-Phe-L-Met-L-Lys-L-Lys-dinitrophenol + dithiothreitol disulfide + H2O
-
 ?