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Literature summary extracted from
- Kinetic characterisation of a dye decolourising peroxidase from Streptomyces lividans new insight into the mechanism of anthraquinone dye decolourisation (2017), Dalton Trans., 46, 9420-9429 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.7 | 0.73 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | pH 7.0, 25°C | Streptomyces lividans |  |
| 1.11.1.7 | 3.1 | - |
K4[Fe(CN)6] | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 5.6 | - |
2,6-dimethyloxyphenol | pH 9.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 10.4 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 15.3 | - |
2,6-dimethyloxyphenol | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 16 | - |
veratryl alcohol | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 16.3 | - |
K4[Fe(CN)6] | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 17.5 | - |
guaiacol | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 28.1 | - |
guaiacol | pH 10.5, 25°C | Streptomyces lividans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.7 | Streptomyces lividans | - |
- |
- |
| 1.11.1.7 | Streptomyces lividans SLI4211 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.7 | 2 guaiacol + H2O2 | - |
Streptomyces lividans | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
| 1.11.1.7 | 2 guaiacol + H2O2 | - |
Streptomyces lividans SLI4211 | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
| 1.11.1.7 | 2 K4[Fe(CN)6] + H2O2 | - |
Streptomyces lividans | K3[Fe(CN)6] + H2O + K2O | - |
? | |
| 1.11.1.7 | 2 K4[Fe(CN)6] + H2O2 | - |
Streptomyces lividans SLI4211 | K3[Fe(CN)6] + H2O + K2O | - |
? | |
| 1.11.1.7 | 2 veratryl alcohol + H2O2 | - |
Streptomyces lividans | 2 veratraldehyde + H2O | - |
? | |
| 1.11.1.7 | 2 veratryl alcohol + H2O2 | - |
Streptomyces lividans SLI4211 | 2 veratraldehyde + H2O | - |
? | |
| 1.11.1.7 | 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | - |
Streptomyces lividans | ? | - |
? | |
| 1.11.1.7 | 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | - |
Streptomyces lividans SLI4211 | ? | - |
? | |
| 1.11.1.7 | 2,6-dimethyloxyphenol + H2O2 | - |
Streptomyces lividans | ? + H2O | - |
? | |
| 1.11.1.7 | additional information | for most substrates, the value of kcat/Km measured by steady-state kinetics is equal to the slowest step in catalysis measured by stopped-flow spectroscopy, namely the decay of the ferryl FeIV=O species (compound II) to form the ferric species | Streptomyces lividans | ? | - |
? | |
| 1.11.1.7 | additional information | for most substrates, the value of kcat/Km measured by steady-state kinetics is equal to the slowest step in catalysis measured by stopped-flow spectroscopy, namely the decay of the ferryl FeIV=O species (compound II) to form the ferric species | Streptomyces lividans SLI4211 | ? | - |
? | |
| 1.11.1.7 | reactive blue 19 + H2O2 | - |
Streptomyces lividans | ? + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.7 | DtpA | - |
Streptomyces lividans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.7 | 0.054 | - |
guaiacol | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.1 | - |
2,6-dimethyloxyphenol | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.32 | - |
2,6-dimethyloxyphenol | pH 9.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.4 | - |
guaiacol | pH 10.5, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.5 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.82 | - |
veratryl alcohol | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 1.12 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 109 | - |
K4[Fe(CN)6] | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 295 | - |
K4[Fe(CN)6] | pH 5.0, 25°C | Streptomyces lividans | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.7 | heme | spectra are typical of high valent heme iron intermediate states generated in the catalytic cycle of a peroxidase | Streptomyces lividans | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.7 | 0.0031 | - |
guaiacol | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.0065 | - |
2,6-dimethyloxyphenol | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.014 | - |
guaiacol | pH 10.5, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.048 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.051 | - |
veratryl alcohol | pH 5.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 0.057 | - |
2,6-dimethyloxyphenol | pH 9.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 1.53 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 6.7 | - |
K4[Fe(CN)6] | pH 7.0, 25°C | Streptomyces lividans | |
| 1.11.1.7 | 95 | - |
K4[Fe(CN)6] | pH 5.0, 25°C | Streptomyces lividans | |
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