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Literature summary extracted from

  • Wang, T.; Zuo, R.; Mai, K.; Xu, W.; Ai, Q.
    Molecular cloning and functional characterization of arachidonate 5-lipoxygenase (Alox5), and its expression in response to the ratio of linolenic acid to linoleic acid in diets of large yellow croaker (Larmichthys crocea) (2016), Comp. Biochem. Physiol. B, 201, 21-28 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 gene Alox5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative realtime PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Larimichthys crocea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.34 (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate Larimichthys crocea epoxidation (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O
-
 ?
1.13.11.34 arachidonate + O2 Larimichthys crocea hydroperoxidation (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Larimichthys crocea A0A075C1I3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Larimichthys crocea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 brain
-
 Larimichthys crocea
-
1.13.11.34 heart
-
 Larimichthys crocea
-
1.13.11.34 intestine
-
 Larimichthys crocea
-
1.13.11.34 kidney high expression level. In the kidney, the expression levels of Alox5 of fish fed diets with low dietary linolenic acid/linoleic acid (0.03-0.06) are significantly higher than those of fish fed diets with high dietary linolenic acid/linoleic acid (0.45-1.51) Larimichthys crocea
-
1.13.11.34 liver in the liver, the Alox5 mRNA expression levels significantly increase to the maximum when the dietary linolenic acid/linoleic acid increases from 0.03 to 0.06, and then significantly decreases with dietary linolenic acid/linoleic acid increased to 1.51 Larimichthys crocea
-
1.13.11.34 muscle low expression level Larimichthys crocea
-
1.13.11.34 spleen
-
 Larimichthys crocea
-
1.13.11.34 stomach
-
 Larimichthys crocea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate epoxidation Larimichthys crocea (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O
-
 ?
1.13.11.34 arachidonate + O2 hydroperoxidation Larimichthys crocea (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.34 ? x * 78060, sequence calculation Larimichthys crocea

Synonyms

EC Number Synonyms Comment Organism
1.13.11.34 ALOX5
-
 Larimichthys crocea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.34 Larimichthys crocea sequence calculation
-
 5.83

Expression

EC Number Organism Comment Expression
1.13.11.34 Larimichthys crocea in the liver, the Alox5 mRNA expression levels significantly increase to the maximum when the dietary linolenic acid/linoleic acid increases from 0.03 to 0.06, and then significantly decreases with dietary linolenic acid/linoleic acid increased to 1.51. In the kidney, the expression levels of Alox5 of fish fed diets with low dietary linolenic acid/linoleic acid (0.03-0.06) are significantly higher than those of fish fed diets with high dietary linolenic acid/linoleic acid (0.45-1.51) up