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Literature summary extracted from

  • Ferreira-Nozawa, M.S.; Rezende, J.L.; Guimaraes, L.H.; Terenzi, H.F.; Jorge, J.A.; Polizeli, M.L.
    Mycelial glucoamylases produced by the thermophilic fungus Scytalidium thermophilum strains 15.1 and 15.8 purification and biochemical characterization (2008), Braz. J. Microbiol., 39, 344-352 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 2-mercaptoethanol 40% residual activity; no residual activity Mycothermus thermophilus
3.2.1.3 Ca2+ 5 mM, 78% residual activity Mycothermus thermophilus
3.2.1.3 Cu2+ 5 mM, 48% residual activity Mycothermus thermophilus
3.2.1.3 Hg2+ 5 mM, 7% residual activity; 5 mM, no residual activity Mycothermus thermophilus
3.2.1.3 Mn2+ 5 mM, 59% residual activity Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 maltose Km value 0.029 mg/ml, pH 5.5, temperature not specified in the publication Mycothermus thermophilus
3.2.1.3 additional information
-
 starch Km value 0.094 mg/ml, pH 5.5, temperature not specified in the publication Mycothermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ca2+ 5 mM, 109% residual activity Mycothermus thermophilus
3.2.1.3 Cu2+ 5 mM, 123% of initial activity Mycothermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 54000
-
 gel filtration Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Mycothermus thermophilus
-
-
-
3.2.1.3 Mycothermus thermophilus 15.1
-
-
-
3.2.1.3 Mycothermus thermophilus 15.8
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein 20% carbohydrate content Mycothermus thermophilus
3.2.1.3 glycoprotein 50% carbohydrate content Mycothermus thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Mycothermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 9.4
-
 substrate starch, pH 5.5, temperature not specified in the publication Mycothermus thermophilus
3.2.1.3 43.8
-
 substrate starch, pH 5.5, temperature not specified in the publication Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 maltose + H2O
-
 Mycothermus thermophilus 2 D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Mycothermus thermophilus 15.8 2 D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Mycothermus thermophilus 15.1 2 D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Mycothermus thermophilus D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Mycothermus thermophilus 15.8 D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Mycothermus thermophilus 15.1 D-glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
-
 Mycothermus thermophilus
3.2.1.3 60
-
-
 Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 60
-
 half-life more than 60 min Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5
-
-
 Mycothermus thermophilus
3.2.1.3 5.5 6
-
 Mycothermus thermophilus