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Literature summary extracted from

  • Chalansonnet, V.; Mercier, C.; Orenga, S.; Gilbert, C.
    Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity (2017), BMC Microbiol., 17, 126 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 gene EF_0404, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain XL1-Blue from vector pQE30 Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Enterococcus faecalis Q838N5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6 recombinant FMN cofactor-bound His-tagged enzyme from Escherichia coli strain XL1-Blue by affinity chromatography and dialysis Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 4-dimethylaminoazobenzene-2'-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis anthranilate + N,N'-dimethylamino-aniline + 2 NADP+
-
 ?
1.7.1.6 additional information the enzyme can also catalyze the nitroreduction of 7-nitrocoumarin-3-carboxylic acid in a FMN-dependent manner Enterococcus faecalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 ? x * 25000, recombinant His-tagged enzyme, SDS-PAGE Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 azoreductase
-
 Enterococcus faecalis
1.7.1.6 EF0404
-
 Enterococcus faecalis
1.7.1.6 EF_0404
-
 Enterococcus faecalis
1.7.1.6 FMN-dependent NAD(P)H nitroreductase
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 35
-
 assay at Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.6 59
-
 Tm of gene EF_0404 Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 7
-
 assay at Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FMN enzyme-bound prosthetic group, FMN-dependence for azoreductase activity Enterococcus faecalis
1.7.1.6 additional information the enzyme can use both cofactors, NADH and NADPH, it shows a preference towards NADH for the azoreductase activity, but a preference towards NADPH for the nitroreductase activity Enterococcus faecalis
1.7.1.6 NADH
-
 Enterococcus faecalis
1.7.1.6 NADPH
-
 Enterococcus faecalis

General Information

EC Number General Information Comment Organism
1.7.1.6 evolution enzyme EF0404 shows both nitroreductase and azoreductase activity. The biochemical characteristics, such as substrate and cofactor specificity, of enzyme EF0404 resemble the properties of the known azoreductase AzoA. But its sequence matches with the nitroreductase group B, the same as enzyme EF0648 Enterococcus faecalis
1.7.1.6 physiological function enzyme EF0404 shows both nitroreductase and azoreductase activity. The biochemical characteristics, such as substrate and cofactor specificity, of enzyme EF0404 resemble the properties of the known azoreductase AzoA. But its sequence matches with the nitroreductase group, the same as enzyme EF0648 Enterococcus faecalis