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Literature summary extracted from

  • Feldman, D.; Kowbel, D.J.; Glass, N.L.; Yarden, O.; Hadar, Y.
    Detoxification of 5-hydroxymethylfurfural by the Pleurotus ostreatus lignolytic enzymes aryl alcohol oxidase and dehydrogenase (2015), Biotechnol. Biofuels, 8, 63 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.3.47 biofuel production current large-scale pretreatment processes for lignocellulosic biomass are generally accompanied by the formation of toxic degradation products, such as 5-hydroxymethylfurfural (HMF), which inhibit cellulolytic enzymes and fermentation by ethanol-producing yeast. Overcoming these toxic effects is a key technical barrier in the biochemical conversion of plant biomass to biofuels. Pleurotus ostreatus, a white-rot fungus, can efficiently degrade lignocellulose, and it can tolerate and metabolize HMF involving HMF oxidase (HMFO) encoded by HmfH Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.7 extracellular
-
 Pleurotus ostreatus
-
-
1.1.3.47 extracellular
-
 Pleurotus ostreatus
-
-
1.1.3.47 extracellular the enzyme is secreted Pleurotus ostreatus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.47 2,5-diformylfuran + 2 O2 Pleurotus ostreatus
-
 2,5-furandicarboxylic acid + H2O2
-
 ?
1.1.3.47 2,5-diformylfuran + 2 O2 Pleurotus ostreatus PC9
-
 2,5-furandicarboxylic acid + H2O2
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2 Pleurotus ostreatus
-
 2,5-diformylfuran + H2O2
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2 Pleurotus ostreatus PC9
-
 2,5-diformylfuran + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Pleurotus ostreatus E5CZV8 cf. EC 1.1.3.47
-
1.1.3.47 Pleurotus ostreatus E5CZV8
-
-
1.1.3.47 Pleurotus ostreatus PC9 E5CZV8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus 2,5-furandicarboxylic acid + ?
-
 ?
1.1.3.7 5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus PC9 2,5-furandicarboxylic acid + ?
-
 ?
1.1.3.7 veratryl alcohol + O2
-
 Pleurotus ostreatus veratraldehyde + H2O2
-
 ?
1.1.3.7 veratryl alcohol + O2
-
 Pleurotus ostreatus PC9 veratraldehyde + H2O2
-
 ?
1.1.3.47 2,5-diformylfuran + 2 O2
-
 Pleurotus ostreatus 2,5-furandicarboxylic acid + H2O2
-
 ?
1.1.3.47 2,5-diformylfuran + 2 O2
-
 Pleurotus ostreatus PC9 2,5-furandicarboxylic acid + H2O2
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus 2,5-diformylfuran + H2O2
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus PC9 2,5-diformylfuran + H2O2
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus 2,5-furandicarboxylic acid + ?
-
 ?
1.1.3.47 5-hydroxymethylfurfural + O2
-
 Pleurotus ostreatus PC9 2,5-furandicarboxylic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 ? x * 75000, SDS-PAGE Pleurotus ostreatus
1.1.3.47 ? x * 75000, SDS-PAGE Pleurotus ostreatus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.47 AAO
-
 Pleurotus ostreatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.47 FAD
-
 Pleurotus ostreatus

Expression

EC Number Organism Comment Expression
1.1.3.7 Pleurotus ostreatus expression is induced in presence of 5-hydroxymethylfurfural up
1.1.3.47 Pleurotus ostreatus 5-hydroxymethylfurfural increases the expression, translation, and activity of enzymes involved in the ligninolytic system, including aryl-alcohol oxidases and a dehydrogenase up
1.1.3.47 Pleurotus ostreatus expression is induced in presence of 5-hydroxymethylfurfural up

General Information

EC Number General Information Comment Organism
1.1.3.7 physiological function Pleurotus ostreatus is capable to metabolize and detoxify 5-hydroxymethylfurfural at 30 mM within 48 h, converting it into 2,5-bis-hydroxymethylfuran and 2,5-furandicarboxylic acid. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidases and a dehydrogenase, are involved in this process. 5-Hydroxymethylfurfural induces the transcription and production of these enzymes, accompanied by an increase in activity levels Pleurotus ostreatus
1.1.3.47 evolution the enzyme is a member of the glucosemethanol-choline oxidase (GMC) oxidoreductase superfamily and the AAO family. Phylogenetic analysis of the AAOs in Pleurotus ostreatous revealed that they cluster into two groups Pleurotus ostreatus
1.1.3.47 physiological function the enzyme is involved in the degradation of 5-hydroxymethylfurfural. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidase and a dehydrogenase, are involved in this process Pleurotus ostreatus
1.1.3.47 physiological function Pleurotus ostreatus is capable to metabolize and detoxify 5-hydroxymethylfurfural at 30 mM within 48 h, converting it into 2,5-bis-hydroxymethylfuran and 2,5-furandicarboxylic acid. Two enzymes groups, which belong to the ligninolytic system, aryl-alcohol oxidases and a dehydrogenase, are involved in this process. 5-Hydroxymethylfurfural induces the transcription and production of these enzymes, accompanied by an increase in activity levels Pleurotus ostreatus