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Literature summary extracted from

  • Karande, R.; Salamanca, D.; Schmid, A.; Buehler, K.
    Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120 (2017), Biotechnol. Bioeng., 115, 312-320 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.245 synthesis synthesis of lactones from cycloalkanes. A heterologous pathway comprising enzymes with compatible kinetics is designed in Pseudomonas taiwanensis VLB120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. The respective pathway includes cytochrome P450 monooxygenase (CHX), cyclohexanol dehydrogenase (CDH), and cyclohexanone monooxygenase (CHXON) from Acidovorax sp. CHX100. Resting cells of the recombinant host Pseudomonas taiwanensis VLB120 convert cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/g cell dry weight, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 h without accumulation of intermediate products Acidovorax sp.
1.14.13.22 synthesis synthesis od lactones from cycloalkanes. A heterologous pathway comprising enzymes with compatible kinetics is designed in Pseudomonas taiwanensis VLB120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. The respective pathway includes cytochrome P450 monooxygenase (CHX), cyclohexanol dehydrogenase (CDH), and cyclohexanone monooxygenase (CHXON) from Acidovorax sp. CHX100. Resting cells of the recombinant host Pseudomonas taiwanensis VLB120 convert cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/g cell dry weight, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 h without accumulation of intermediate products Acidovorax sp. CHX100
1.14.13.22 synthesis synthesis of lactones from cycloalkanes in Pseudomonas taiwanensis VLB120. The pathway includes cytochrome P450 monooxygenase, cyclohexanol dehydrogenase, and cyclohexanone monooxygenase from Acidovorax sp. CHX100. Recombinant P. taiwanensis converts cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/gCDW, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 hr without accumulation of intermediate products. Analogous lactones can be obtained from cyclooctane and cyclodecane Acidovorax sp. CHX100

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.245 expression in Pseudomonas taiwanensis VLB120 Acidovorax sp.
1.14.13.22 expression in Pseudomonas taiwanensis VLB120 Acidovorax sp. CHX100

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.245 Acidovorax sp. A0A2D0WG37
-
-
1.14.13.22 Acidovorax sp. CHX100 A0A2D0WG32
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.245 CHXON
-
 Acidovorax sp.
1.14.13.22 CHXON
-
 Acidovorax sp. CHX100