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Literature summary extracted from
- Expression and characterization of Streptomyces coelicolor serine/threonine protein kinase PkaE (2015), Biosci. Biotechnol. Biochem., 79, 855-862 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.1 | additional information | recombinant PkaE is activated by autophosphorylation at threonine residues only, but not serine or tyrosine residues | Streptomyces coelicolor |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.11.1 | gene pkaE, recombinant expression of GST-tagged wild-type enzyme and mutant enzymes, and of His-tagged enzyme mutant K42N in Escherichia coli strain BL21(DE3)plysS | Streptomyces coelicolor |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | K42N | site-directed mutagenesis, inactive mutant | Streptomyces coelicolor |
| 2.7.11.1 | K42Q | site-directed mutagenesis, inactive mutant | Streptomyces coelicolor |
| 2.7.11.1 | additional information | construction of a gene disruption mutant of pkaE | Streptomyces coelicolor |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.11.1 | cytoplasm | - |
Streptomyces coelicolor | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.1 | Mg2+ | required | Streptomyces coelicolor |  |
| 2.7.11.1 | Mn2+ | required | Streptomyces coelicolor | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.11.1 | Streptomyces coelicolor | Q9ZNB6 | - |
- |
| 2.7.11.1 | Streptomyces coelicolor A3(2) M145 | Q9ZNB6 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | phosphoprotein | recombinant PkaE autophosphorylates at threonine residues only, but not serine or tyrosine residues | Streptomyces coelicolor |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.11.1 | recombinant GST-tagged wild-type enzyme and mutant enzymes, and His-tagged enzyme mutant K42N from Escherichia coli strain BL21(DE3)plysS by glutathione or nickel affinity chromatography, and gel filtration | Streptomyces coelicolor |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.11.1 | additional information | gene pkaE is expressed during the late growth phase in Streptomyces coelicolor A3 (2) M145, which corresponds to the production time of blue pigments, pkaE is expressed during the late growth phase (60 to 84 h), but not the early growth phase (36 to 48 h) | Streptomyces coelicolor | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.11.1 | ATP + KbpA | recombinant His-tagged substrate, 28 kDa | Streptomyces coelicolor | ADP + phospho-KbpA | - |
? | |
| 2.7.11.1 | ATP + KbpA | recombinant His-tagged substrate, 28 kDa | Streptomyces coelicolor A3(2) M145 | ADP + phospho-KbpA | - |
? | |
| 2.7.11.1 | additional information | recombinant PkaE autophosphorylates at threonine residues only, but not serine or tyrosine residues | Streptomyces coelicolor | ? | - |
? | |
| 2.7.11.1 | additional information | recombinant PkaE autophosphorylates at threonine residues only, but not serine or tyrosine residues | Streptomyces coelicolor A3(2) M145 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | ? | x * 89000-94000, recombinant GST-tagged wild-type autophosphorylated enzyme PkaE, SDS-PAGE, x * 79500-80000, recombinant GST-tagged mutant enzymes, SDS-PAGE | Streptomyces coelicolor |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | PkaE | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.11.1 | 30 | - |
assay at | Streptomyces coelicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.11.1 | 7.2 | - |
assay at | Streptomyces coelicolor |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.1 | ATP | - |
Streptomyces coelicolor | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | evolution | the N-terminal region of PkaE is similar to the catalytic domains of several bacterial serine/ threonine protein kinases. In contrast, the C-terminal region of PkaE has no significant sequence similarity to any proteins | Streptomyces coelicolor |
| 2.7.11.1 | malfunction | disruption of chromosomal pkaE results in the overproduction of the actinorhodin-related blue pigment antibiotics | Streptomyces coelicolor |
| 2.7.11.1 | physiological function | enzyme PkaE acts as a negative regulator for production of the secondary metabolites. In addition, PkaE is able to phosphorylate KbpA, a regulator involved in the AfsKAfsR regulatory pathway | Streptomyces coelicolor |
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