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Literature summary extracted from

  • Trindade, L.V.; Desagiacomo, C.; Polizeli, M.L.; Damasio, A.R.; Lima, A.M.; Gomes, E.; Bonilla-Rodriguez, G.O.
    Biochemical characterization, thermal stability, and partial sequence of a novel exo-polygalacturonase from the thermophilic fungus Rhizomucor pusillus A13.36 obtained by submerged cultivation (2016), BioMed Res. Int., 2016, 8653583 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ba2+ 10 mM, 24% residual activity; 24.5% residual activity at 10 mM Rhizomucor pusillus
3.2.1.67 Fe2+ complete inhibition at 10 mM Rhizomucor pusillus
3.2.1.67 Fe3+ 10 mM, no residual activity Rhizomucor pusillus
3.2.1.67 Mg2+ 10 mM, 34% residual activity; 34.1% residual activity at 10 mM Rhizomucor pusillus
3.2.1.67 Mn2+ complete inhibition at 10 mM Rhizomucor pusillus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Rhizomucor pusillus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Al3+ 10 mM, 125% of initial activity Rhizomucor pusillus
3.2.1.67 Al3+ 125.4% activity at 10 mM Rhizomucor pusillus
3.2.1.67 Ca2+ 10 mM, 170% of initial activity Rhizomucor pusillus
3.2.1.67 Ca2+ 170.3% activity at 10 mM Rhizomucor pusillus
3.2.1.67 additional information not influenced by Na+ and K+ Rhizomucor pusillus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 43500
-
 gel filtration Rhizomucor pusillus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 citrus pectin + H2O Rhizomucor pusillus citrus pectin with 26% degree of methyl esterification is a better substrate than citrus pectin with 82% degree of methyl esterification galacturonic acid
-
 ?
3.2.1.67 citrus pectin + H2O Rhizomucor pusillus A13.36 citrus pectin with 26% degree of methyl esterification is a better substrate than citrus pectin with 82% degree of methyl esterification galacturonic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Rhizomucor pusillus
-
-
-
3.2.1.67 Rhizomucor pusillus A13.36
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 ammonium sulfate precipitation and HiTrap phenyl column chromatography Rhizomucor pusillus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 171
-
 pH 5.0, 60°C Rhizomucor pusillus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 citric pectin + H2O higher activity of the enzyme on pectin with 26% of esterification compared to 82% esterification Rhizomucor pusillus D-galacturonic acid + ?
-
 ?
3.2.1.67 citric pectin + H2O higher activity of the enzyme on pectin with 26% of esterification compared to 82% esterification Rhizomucor pusillus A13.36 D-galacturonic acid + ?
-
 ?
3.2.1.67 citrus pectin + H2O citrus pectin with 26% degree of methyl esterification is a better substrate than citrus pectin with 82% degree of methyl esterification Rhizomucor pusillus galacturonic acid
-
 ?
3.2.1.67 citrus pectin + H2O citrus pectin with 26% degree of methyl esterification is a better substrate than citrus pectin with 82% degree of methyl esterification Rhizomucor pusillus A13.36 galacturonic acid
-
 ?
3.2.1.67 polygalacturonate
-
 Rhizomucor pusillus galacturonic acid
-
 ?
3.2.1.67 polygalacturonate
-
 Rhizomucor pusillus A13.36 galacturonic acid
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Rhizomucor pusillus galacturonic acid
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Rhizomucor pusillus A13.36 galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 47000, linear regression Rhizomucor pusillus
3.2.1.67 monomer 1 * 47000, SDS-PAGE Rhizomucor pusillus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 Exo-PG
-
 Rhizomucor pusillus
3.2.1.67 exo-polygalacturonanase
-
 Rhizomucor pusillus
3.2.1.67 pectinase
-
 Rhizomucor pusillus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 60
-
-
 Rhizomucor pusillus
3.2.1.67 61
-
-
 Rhizomucor pusillus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 30 60
-
 Rhizomucor pusillus
3.2.1.67 30 60 thermal stability between 30 and 60°C with more than 70% activity remaining after 24 h Rhizomucor pusillus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 4
-
-
 Rhizomucor pusillus
3.2.1.67 4
-
 purified enzyme Rhizomucor pusillus
3.2.1.67 4.5
-
 crude enzyme preparation Rhizomucor pusillus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 3.5 9.5
-
 Rhizomucor pusillus
3.2.1.67 3.5 9.5 stable in pH ranging from 3.5 to 9.5 with more than 60% activity remaining after 24 h Rhizomucor pusillus