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Literature summary extracted from

  • Schroeder, M.; Haefner, A.K.; Hofmann, B.; Radmark, O.; Tumulka, F.; Abele, R.; Doetsch, V.; Steinhilber, D.
    Stabilisation and characterisation of the isolated regulatory domain of human 5-lipoxygenase (2014), Biochim. Biophys. Acta, 1842, 1538-1547 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.34 medicine the enzyme is a promising target for anti-inflammatory therapy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 recombinant expression of His-tagged wild-type enzyme, enzyme mutant, and isolated enzyme PLAT domain in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.34 additional information the isolated PLAT W75G interacts with calcium and the Dicer fragment Homo sapiens
1.13.11.34 W75G site-directed mutagenesis, the mutation causes reduced aggregation and substantially increased thermal stability of the mutant. The mutant still shows Ca2+ interactions. Mutation W75G thermally stabilises the isolated PLAT domain Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.34 cytosol
-
 Homo sapiens 5829
-
1.13.11.34 additional information membrane-binding residue tryptophan 75 Homo sapiens
-
-
1.13.11.34 nuclear membrane
-
 Homo sapiens 31965
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ca2+ allosteric modulator involved in membrane binding by the enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.34 13800
-
 isolated enzyme PLAT domain, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.34 arachidonate + O2 Homo sapiens
-
 (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Homo sapiens P09917
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 recombinant His-tagged wild-type enzyme and enzyme mutant from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, isolated enzyme PLAT domain Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 arachidonate + O2
-
 Homo sapiens (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.34 5-lipoxygenase
-
 Homo sapiens
1.13.11.34 5-LOX
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.34 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.34 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
1.13.11.34 additional information the ability of the isolated PLAT domain to bind Dicer C-terminus whereas the interaction with coactosin-like protein requires the interplay of the catalytic and the PLAT domain Homo sapiens
1.13.11.34 physiological function 5-lipoxygenase (5-LOX) is the key player of pro-inflammatory leukotriene biosynthesis. Its regulatory or socalled PLAT (polycystin-1, lipoxygenase, alpha-toxin) domain binds allosteric modulators like calcium, membranes, coactosin-like protein and Dicer, thereby influencing 5-LOX activity at the nuclearmembrane by mediating translocation. The PLAT domain may also regulate cytosolic 5-LOX activity and possibly influence microRNA metabolism Homo sapiens