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Literature summary extracted from

  • Rezessy-Szabo, J.M.; Nguyen, Q.D.; Hoschke, A.; Braet, C.; Hajos, G.; Claeyssens, M.
    A novel thermostable alpha-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b purification and characterization (2007), Biochim. Biophys. Acta, 1770, 55-62 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ 10 mM, complete inactivation Thermomyces lanuginosus
3.2.1.22 Ca2+
-
 Thermomyces lanuginosus
3.2.1.22 Hg2+
-
 Thermomyces lanuginosus
3.2.1.22 Zn2+
-
 Thermomyces lanuginosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 1.13
-
 4-nitrophenyl alpha-D-galactopyranoside pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus
3.2.1.22 1.17
-
 stachyose pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus
3.2.1.22 1.61
-
 raffinose pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.22 extracellular
-
 Thermomyces lanuginosus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Mn2+
-
 Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Thermomyces lanuginosus
-
-
-
3.2.1.22 Thermomyces lanuginosus CBS 395.62/b
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.22 glycoprotein carbohydrate content 5.3% Thermomyces lanuginosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Thermomyces lanuginosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 culture fluid
-
 Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 1813
-
 pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus

Storage Stability

EC Number Storage Stability Organism
3.2.1.22 -20°C, 4-5 days, 60-70% loss of activity Thermomyces lanuginosus
3.2.1.22 4°C, 4-5 days, 60-70% loss of activity Thermomyces lanuginosus
3.2.1.22 highly stable in freeze-dried form or in buffered solutions containing 10-20% (w/v) NaCl Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermomyces lanuginosus 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermomyces lanuginosus CBS 395.62/b 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Thermomyces lanuginosus D-galactose + sucrose
-
 ?
3.2.1.22 raffinose + H2O
-
 Thermomyces lanuginosus CBS 395.62/b D-galactose + sucrose
-
 ?
3.2.1.22 stachyose + H2O
-
 Thermomyces lanuginosus raffinose + D-galactose
-
 ?
3.2.1.22 stachyose + H2O
-
 Thermomyces lanuginosus CBS 395.62/b raffinose + D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 monomer 1 * 93000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 65
-
-
 Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 55
-
 pH 6.4-8.3, stable for at least 24 h Thermomyces lanuginosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 3914
-
 4-nitrophenyl alpha-D-galactopyranoside pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus
3.2.1.22 6946
-
 raffinose pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus
3.2.1.22 7659
-
 stachyose pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5 5.5
-
 Thermomyces lanuginosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 6.4 8.3 55°C, stable for at least 24 h Thermomyces lanuginosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Thermomyces lanuginosus isoelectric focusing
-
 3.9

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.22 3455
-
 4-nitrophenyl alpha-D-galactopyranoside pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus
3.2.1.22 4305
-
 raffinose pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus
3.2.1.22 6555
-
 stachyose pH not specified in the publication, temperature not specified in the publication Thermomyces lanuginosus