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Importance of hydrogen bonding in fine tuning the [2Fe-2S] cluster redox potential of HydC from Thermotoga maritima

Birrell, J.A.; Laurich, C.; Reijerse, E.J.; Ogata, H.; Lubitz, W.; Biochemistry 55, 4344-4355 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.12.1.4
expressed in Escherichia coli BL21(DE3) cells
Thermotoga maritima
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.12.1.4
A85P
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
1.12.1.4
V131N
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima
-
H+ + NADH + reduced ferredoxin
-
-
?
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
H+ + NADH + reduced ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.1.4
Thermotoga maritima
-
-
-
1.12.1.4
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.1.4
streptactin column chromatography
Thermotoga maritima
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima
H+ + NADH + reduced ferredoxin
-
-
-
?
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
H+ + NADH + reduced ferredoxin
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.4
[2Fe-2S]-center
-
Thermotoga maritima
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.1.4
expressed in Escherichia coli BL21(DE3) cells
Thermotoga maritima
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.4
[2Fe-2S]-center
-
Thermotoga maritima
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.12.1.4
A85P
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
1.12.1.4
V131N
the mutant has an altered reduction potential compared to the wild type enzyme
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima
-
H+ + NADH + reduced ferredoxin
-
-
?
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
H+ + NADH + reduced ferredoxin
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.1.4
streptactin column chromatography
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima
H+ + NADH + reduced ferredoxin
-
-
-
?
1.12.1.4
H2 + NAD+ + oxidized ferredoxin
-
741931
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
H+ + NADH + reduced ferredoxin
-
-
-
?