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Literature summary extracted from
- Crystal structure and characterization of a novel L-serine ammonia-lyase from Rhizomucor miehei (2015), Biochem. Biophys. Res. Commun., 466, 431-437 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.1.17 | expression in Pichia pastoris | Rhizomucor miehei |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.3.1.17 | to 1.76 A resolution. The active site is located between the small domain and large domain, and a pyridoxal 5'-phosphate is attached to Lys52 by a Schiff-base linkage | Rhizomucor miehei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.17 | Rhizomucor miehei | A0A0X1KHE6 | - |
- |
| 4.3.1.17 | Rhizomucor miehei CAU432 | A0A0X1KHE6 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.17 | 7.35 | - |
pH 7.5, 40°C | Rhizomucor miehei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.17 | L-serine | - |
Rhizomucor miehei | pyruvate + NH3 | - |
? |  |
| 4.3.1.17 | L-serine | - |
Rhizomucor miehei CAU432 | pyruvate + NH3 | - |
? | |
| 4.3.1.17 | L-threonine | - |
Rhizomucor miehei | 2-oxobutanoate + NH3 | - |
? | |
| 4.3.1.17 | L-threonine | - |
Rhizomucor miehei CAU432 | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.1.17 | ? | * 41000, SDS-PAGE | Rhizomucor miehei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.17 | 40 | - |
- |
Rhizomucor miehei |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.17 | 40 | - |
stable below | Rhizomucor miehei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.17 | 7.5 | - |
- |
Rhizomucor miehei |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.17 | 7 | 9 | - |
Rhizomucor miehei |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.17 | pyridoxal 5'-phosphate | cofactor is located in the crevice between the two domains, attached to residue Lys52 by a Schiff-base linkage | Rhizomucor miehei | |
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Release 2023.1 (January 2023)
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