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Literature summary extracted from

  • Couturier, M.; Mathieu, Y.; Li, A.; Navarro, D.; Drula, E.; Haon, M.; Grisel, S.; Ludwig, R.; Berrin, J.G.
    Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis (2016), Appl. Microbiol. Biotechnol., 100, 697-706 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 phylogenetic analysis, recombinant expression in Pichia pastoris Ustilago maydis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.7 4-Hydroxybenzyl alcohol shows substrate inhibition at higher concentration Ustilago maydis
1.1.3.7 anisyl alcohol shows substrate inhibition at higher concentration Ustilago maydis
1.1.3.7 cinnamyl alcohol shows substrate inhibition at higher concentration Ustilago maydis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 additional information
-
 additional information Michaelis-Menten kinetics Ustilago maydis
1.1.3.7 0.0048
-
 anisyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 0.015
-
 2,4-hexadien-1-ol pH 6.0, 30°C Ustilago maydis
1.1.3.7 0.035
-
 cinnamyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 0.042
-
 4-Hydroxybenzyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 0.12
-
 veratryl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 1.82
-
 2,4-Dimethoxybenzyl alcohol pH 6.0, 30°C Ustilago maydis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.7 extracellular the enzyme is secreted Ustilago maydis
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Ustilago maydis
-
 strains BRFM 1093 and 521/FGSC 9021
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.7 additional information the enzyme contains no putative N/O-glycosylation sites Ustilago maydis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.7 additional information Ustilago maydis grown on maize Ustilago maydis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 2,4-dimethoxybenzyl alcohol + O2
-
 Ustilago maydis 2,4-dimethoxybenzaldehyde + H2O2
-
 ?
1.1.3.7 2,4-hexadien-1-ol + O2
-
 Ustilago maydis ? + H2O2
-
 ?
1.1.3.7 4-hydroxybenzyl alcohol + O2
-
 Ustilago maydis 4-hydroxybenzaldehyde + H2O2
-
 ?
1.1.3.7 4-methoxybenzyl alcohol + O2
-
 Ustilago maydis 4-methoxybenzaldehyde + H2O2
-
 ?
1.1.3.7 anisyl alcohol + O2 best substrate Ustilago maydis anisaldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Ustilago maydis benzaldehyde + H2O2
-
 ?
1.1.3.7 cinnamyl alcohol + O2
-
 Ustilago maydis cinnamaldehyde + H2O2
-
 ?
1.1.3.7 additional information the enzyme is able to oxidize several aromatic alcohols. Of the tested aryl-alcohols, the highest oxidation rate is obtained with 4-anisyl alcohol. Oxygen, 1,4-benzoquinone, and 2,6-dichloroindophenol can serve as electron acceptors. Assay method using ABTS [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)]/horseradish peroxidase. The enzyme shows no activity as a GMC oxidoreductase Ustilago maydis ?
-
 ?
1.1.3.7 veratryl alcohol + O2 i.e. 3,4-dimethoxybenzyl alcohol Ustilago maydis veratrylaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 More enzyme homology structure modeling Ustilago maydis

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 AAO
-
 Ustilago maydis
1.1.3.7 um04044
-
 Ustilago maydis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.7 30
-
 assay at Ustilago maydis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 30
-
 2,4-Dimethoxybenzyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 45
-
 anisyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 53
-
 veratryl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 64
-
 2,4-hexadien-1-ol pH 6.0, 30°C Ustilago maydis
1.1.3.7 75
-
 4-Hydroxybenzyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 88
-
 cinnamyl alcohol pH 6.0, 30°C Ustilago maydis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 6
-
 assay at Ustilago maydis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD
-
 Ustilago maydis

General Information

EC Number General Information Comment Organism
1.1.3.7 evolution the enzyme belongs to the AA3_2 subfamily, phylogenetic analysis Ustilago maydis
1.1.3.7 additional information enzyme homology structure modeling Ustilago maydis
1.1.3.7 physiological function the aryl-alcohol oxidase acts on the lignin fraction in biomass Ustilago maydis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 16.5
-
 2,4-Dimethoxybenzyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 440
-
 veratryl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 1790
-
 4-Hydroxybenzyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 2510
-
 cinnamyl alcohol pH 6.0, 30°C Ustilago maydis
1.1.3.7 4270
-
 2,4-hexadien-1-ol pH 6.0, 30°C Ustilago maydis
1.1.3.7 9380
-
 anisyl alcohol pH 6.0, 30°C Ustilago maydis