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Literature summary extracted from

  • Jouanneau, Y.; Meyer, C.; Duraffourg, N.
    Dihydroxylation of four- and five-ring aromatic hydrocarbons by the naphthalene dioxygenase from Sphingomonas CHY-1 (2016), Appl. Microbiol. Biotechnol., 100, 1253-1263 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.12 expressed in Escherichia coli BL21(DE3) cells Sphingomonas sp. CHY-1

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.12 Sphingomonas sp. CHY-1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.12 benzo[a]pyrene + NADH + H+ + O2
-
 Sphingomonas sp. CHY-1 cis-9,10-dihydroxy-9,10-dihydrobenzo[a]pyrene + NAD+
-
 ?
1.14.12.12 benz[a]anthracene + NADH + H+ + O2
-
 Sphingomonas sp. CHY-1 cis,cis-1,2,10,11-tetrahydroxy-1,2,10,11-tetrahydrobenz[a]anthracene + NAD+
-
 ?
1.14.12.12 chrysene + NADH + H+ + O2
-
 Sphingomonas sp. CHY-1 cis-3,4-dihydroxy-3,4-dihydrochrysene + NAD+
-
 ?
1.14.12.12 fluoranthene + NADH + H+ + O2
-
 Sphingomonas sp. CHY-1 cis-7,8-dihydroxy-7,8-dihydrofluoranthene + cis-2,3-dihydroxy-2,3-dihydrofluoranthene + NAD+
-
 ?
1.14.12.12 pyrene + NADH + H+ + O2 poor substrate Sphingomonas sp. CHY-1 pyrene cis-4,5-dihydrodiol + NAD+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.12 NADH
-
 Sphingomonas sp. CHY-1