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Literature summary extracted from

  • Gao, X.; Zhang, Z.; Zhang, Y.; Li, Y.; Zhu, H.; Wang, S.; Li, C.
    A newly determined member of the meso-diaminopimelate dehydrogenase family with a broad substrate spectrum (2017), Appl. Environ. Microbiol., 83, e00476 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.16 expressed in Escherichia coli BL21(DE3) cells Symbiobacterium thermophilum

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.16 R35S the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 R71A the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme Symbiobacterium thermophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.1
-
NADPH wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.18
-
NADPH mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.35
-
NADPH mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.35
-
NADP+ wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.39
-
meso-2,6-diaminoheptanedioate mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.39
-
NADP+ mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.41
-
meso-2,6-diaminoheptanedioate wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.42
-
meso-2,6-diaminoheptanedioate mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.46
-
NADP+ mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 7.45
-
pyruvate mutant enzyme R35S, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 16.31
-
pyruvate wild type enzyme, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 21.77
-
pyruvate mutant enzyme R71A, at pH 8.6 and 25°C Symbiobacterium thermophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ Symbiobacterium thermophilum
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ Symbiobacterium thermophilum IAM14863
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Symbiobacterium thermophilum
-
-
-
1.4.1.16 Symbiobacterium thermophilum IAM14863
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 2-oxosuccinic acid + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum ?
-
?
1.4.1.16 2-oxosuccinic acid + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum IAM14863 ?
-
?
1.4.1.16 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum ?
-
?
1.4.1.16 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum IAM14863 ?
-
?
1.4.1.16 4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ low activity Symbiobacterium thermophilum ?
-
?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ highest activity Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
Symbiobacterium thermophilum IAM14863 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ highest activity Symbiobacterium thermophilum IAM14863 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
r
1.4.1.16 pyruvate + NADPH
-
Symbiobacterium thermophilum ?
-
?
1.4.1.16 pyruvate + NADPH
-
Symbiobacterium thermophilum IAM14863 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.16 meso-DAPDH
-
Symbiobacterium thermophilum
1.4.1.16 meso-diaminopimelate dehydrogenase
-
Symbiobacterium thermophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.16 2.92
-
NADPH mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 2.96
-
pyruvate mutant enzyme R35S, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 3.31
-
pyruvate mutant enzyme R71A, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 4.17
-
NADPH mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 6.97
-
NADPH wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 7
-
pyruvate wild type enzyme, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 17.91
-
NADP+ mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 25.47
-
NADP+ wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 29.44
-
meso-2,6-diaminoheptanedioate mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 40.46
-
meso-2,6-diaminoheptanedioate mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 40.47
-
meso-2,6-diaminoheptanedioate wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 58.16
-
NADP+ mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NADP+
-
Symbiobacterium thermophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.16 0.15
-
pyruvate mutant enzyme R71A, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.4
-
pyruvate mutant enzyme R35S, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 0.43
-
pyruvate wild type enzyme, at pH 8.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 8.32
-
NADPH mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 23.49
-
NADPH mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 39.02
-
NADP+ mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 69.29
-
NADPH wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 73.83
-
NADP+ wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 75.07
-
meso-2,6-diaminoheptanedioate mutant enzyme R35S, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 96.54
-
meso-2,6-diaminoheptanedioate mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 98.48
-
meso-2,6-diaminoheptanedioate wild type enzyme, at pH 9.6 and 25°C Symbiobacterium thermophilum
1.4.1.16 148.06
-
NADP+ mutant enzyme R71A, at pH 9.6 and 25°C Symbiobacterium thermophilum