EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.16 | expressed in Escherichia coli BL21(DE3) cells | Symbiobacterium thermophilum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.16 | R35S | the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme | Symbiobacterium thermophilum |
1.4.1.16 | R71A | the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme | Symbiobacterium thermophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.16 | 0.1 | - |
NADPH | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.18 | - |
NADPH | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.35 | - |
NADPH | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.35 | - |
NADP+ | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.39 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.39 | - |
NADP+ | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.41 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.42 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.46 | - |
NADP+ | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 7.45 | - |
pyruvate | mutant enzyme R35S, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 16.31 | - |
pyruvate | wild type enzyme, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 21.77 | - |
pyruvate | mutant enzyme R71A, at pH 8.6 and 25°C | Symbiobacterium thermophilum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ | Symbiobacterium thermophilum | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ | Symbiobacterium thermophilum IAM14863 | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.16 | Symbiobacterium thermophilum | - |
- |
- |
1.4.1.16 | Symbiobacterium thermophilum IAM14863 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.16 | 2-oxosuccinic acid + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum | ? | - |
? | |
1.4.1.16 | 2-oxosuccinic acid + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
1.4.1.16 | 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum | ? | - |
? | |
1.4.1.16 | 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
1.4.1.16 | 4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum | ? | - |
? | |
1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ | highest activity | Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum IAM14863 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
1.4.1.16 | meso-2,6-diaminoheptanedioate + H2O + NADP+ | highest activity | Symbiobacterium thermophilum IAM14863 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
1.4.1.16 | pyruvate + NADPH | - |
Symbiobacterium thermophilum | ? | - |
? | |
1.4.1.16 | pyruvate + NADPH | - |
Symbiobacterium thermophilum IAM14863 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.16 | meso-DAPDH | - |
Symbiobacterium thermophilum |
1.4.1.16 | meso-diaminopimelate dehydrogenase | - |
Symbiobacterium thermophilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.16 | 2.92 | - |
NADPH | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 2.96 | - |
pyruvate | mutant enzyme R35S, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 3.31 | - |
pyruvate | mutant enzyme R71A, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 4.17 | - |
NADPH | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 6.97 | - |
NADPH | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 7 | - |
pyruvate | wild type enzyme, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 17.91 | - |
NADP+ | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 25.47 | - |
NADP+ | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 29.44 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 40.46 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 40.47 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 58.16 | - |
NADP+ | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.16 | NADP+ | - |
Symbiobacterium thermophilum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.16 | 0.15 | - |
pyruvate | mutant enzyme R71A, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.4 | - |
pyruvate | mutant enzyme R35S, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 0.43 | - |
pyruvate | wild type enzyme, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 8.32 | - |
NADPH | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 23.49 | - |
NADPH | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 39.02 | - |
NADP+ | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 69.29 | - |
NADPH | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 73.83 | - |
NADP+ | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 75.07 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 96.54 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 98.48 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
1.4.1.16 | 148.06 | - |
NADP+ | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum |