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Literature summary extracted from

  • Pantouris, G.; Serys, M.; Yuasa, H.J.; Ball, H.J.; Mowat, C.G.
    Human indoleamine 2,3-dioxygenase-2 has substrate specificity and inhibition characteristics distinct from those of indoleamine 2,3-dioxygenase-1 (2014), Amino Acids, 46, 2155-2163 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 ascorbic acid required Homo sapiens
1.13.11.52 methylene blue required Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.52 gene IDO1, recombinant expression His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens
1.13.11.52 gene IDO2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.52 additional information generation of a IDO2 construct encoding an N-terminal truncated version of the predicted protein sequence consisting of residues 14-420 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 1-methyl-D-tryptophan competitive inhibition of IDO2, antitumour activity, selectivity of 1-methyltryptophan inhibition of IDO1 and IDO2 Homo sapiens
1.13.11.52 1-methyl-L-tryptophan competitive inhibition of IDO1, antitumour activity, selectivity of 1-methyl L-tryptophan inhibition of IDO1 and IDO2; competitive inhibition of IDO2, antitumour activity, selectivity of 1-methyltryptophan inhibition of IDO1 and IDO2 Homo sapiens
1.13.11.52 D-tryptophan low inhibition Homo sapiens
1.13.11.52 L-tryptophan
-
 Homo sapiens
1.13.11.52 additional information no inhibition of IDO1 by 1-methy-D-tryptophan; no inhibition of IDO1 by L-tryptophan Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens
1.13.11.52 0.006
-
 5-fluoro-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.017
-
 5-hydroxy-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.0209
-
 L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.07
-
 1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.098
-
 5-methyl-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.296
-
 D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.547
-
 5-methoxy-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.66
-
 1-methyl-D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.696
-
 1-methyl-L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.747
-
 1-methyl-D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 1.57
-
 5-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 1.768
-
 5-fluoro-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 3.457
-
 6-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 3.609
-
 D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 6.809
-
 L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.52 L-tryptophan + O2 Homo sapiens
-
 N-formyl-L-kynurenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Homo sapiens P14902
-
-
1.13.11.52 Homo sapiens Q6ZQW0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52 recombinant His-tagged IDO1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens
1.13.11.52 recombinant His-tagged IDO2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.52 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 1-methyl-D-tryptophan + O2
-
 Homo sapiens N-methyl-N-formyl-D-kynurenine
-
 ?
1.13.11.52 1-methyl-L-tryptophan + O2
-
 Homo sapiens N-methyl-N-formyl-L-kynurenine
-
 ?
1.13.11.52 5-fluoro-DL-tryptophan + O2
-
 Homo sapiens 5-fluoro-N-formyl-DL-kynurenine
-
 ?
1.13.11.52 5-hydroxy-L-tryptophan + O2
-
 Homo sapiens 5-hydroxy-N-formyl-L-kynurenine
-
 ?
1.13.11.52 5-methoxy-DL-tryptophan + O2
-
 Homo sapiens 5-methoxy-N-formyl-DL-kynurenine
-
 ?
1.13.11.52 5-methyl-DL-tryptophan + O2
-
 Homo sapiens 5-methyl-N-formyl-DL-kynurenine
-
 ?
1.13.11.52 5-methyl-DL-tryptophan + O2 high activity Homo sapiens 5-methyl-N-formyl-DL-kynurenine
-
 ?
1.13.11.52 6-methyl-DL-tryptophan + O2
-
 Homo sapiens 6-methyl-N-formyl-DL-kynurenine
-
 ?
1.13.11.52 D-tryptophan + O2
-
 Homo sapiens N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?
1.13.11.52 additional information substrate specificity, overview. Replacement of the 5-methoxy group by a methyl group has a negative influence in the binding of the molecule, decreasing its affinity threefold. 5-HO-L-Trp and melatonin are no substrates for IDO2 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.52 IDO1
-
 Homo sapiens
1.13.11.52 IDO2
-
 Homo sapiens
1.13.11.52 indoleamine 2,3-dioxygenase-1
-
 Homo sapiens
1.13.11.52 indoleamine 2,3-dioxygenase-2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.52 25
-
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.52 0.0052
-
 1-methyl-D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.0103
-
 D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.011
-
 1-methyl-L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.025
-
 5-hydroxy-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.049
-
 5-fluoro-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.062
-
 1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.095
-
 1-methyl-D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.103
-
 L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.108
-
 6-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.161
-
 5-methoxy-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.36
-
 5-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.76
-
 5-fluoro-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 2.7
-
 D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 2.97
-
 L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 3.78
-
 5-methyl-DL-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.52 6.5
-
-
 Homo sapiens
1.13.11.52 7.5
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 heme
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.52 0.018
-
 1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.05
-
 L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 0.3
-
 1-methyl-L-tryptophan pH 6.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.543
-
 D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO1 Homo sapiens
1.13.11.52 3.3
-
 1-methyl-D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 12
-
 D-tryptophan pH 6.5, 25°C, recombinant enzyme IDO2 Homo sapiens

General Information

EC Number General Information Comment Organism
1.13.11.52 metabolism indoleamine 2,3-dioxygenase-2 (IDO2) is one of three enzymes, alongside tryptophan 2,3-dioxygenase (EC 1.13.11.11) and indoleamine 2,3-dioxygenase (IDO1), that catalyse dioxygenation of L-tryptophan as the first step in the kynurenine pathway Homo sapiens
1.13.11.52 additional information human indoleamine 2,3-dioxygenase-2 substrate specificity and inhibition characteristics are distinct from those of indoleamine 2,3-dioxygenase-1 Homo sapiens
1.13.11.52 physiological function the enzyme IDO2 might have an immunomodulatory role unrelated to that of IDO1 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.52 0.0028
-
 D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.007
-
 1-methyl-D-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.015
-
 L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.016
-
 1-methyl-L-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.028
-
 5-fluoro-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.031
-
 6-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.229
-
 5-methyl-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens
1.13.11.52 0.294
-
 5-methoxy-DL-tryptophan pH 7.5, 25°C, recombinant enzyme IDO2 Homo sapiens