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Literature summary extracted from

  • Cereia, M.; Guimaraes, L.; Peixoto-Nogueira, S.; Jorge, J.; Terenzi, H.; Greene, L.; Polizeli, M.
    Glucoamylase isoform (GAII) purified from a thermophilic fungus Scytalidium thermophilum 15.8 with biotechnological potential (2006), Afr. J. Biotechnol., 5, 1239-1245 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 2-mercaptoethanol 1 mM, complete loss of activity Mycothermus thermophilus
3.2.1.3 Cu2+ 10 mM, 71% residual activity with substrate starch, 31% with substrate maltose Mycothermus thermophilus
3.2.1.3 Hg2+ 10 mM, 10% residual activity Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 starch Km value 0.2 mg/ml, pH 5.5, 60°C Mycothermus thermophilus
3.2.1.3 additional information
-
 maltose Km value 1.5 mg/ml, pH 5.5, 60°C Mycothermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Mycothermus thermophilus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Mycothermus thermophilus
-
-
-
3.2.1.3 Mycothermus thermophilus 15.8
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein 10% carbohydrate content Mycothermus thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 maltose + H2O
-
 Mycothermus thermophilus 2 D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Mycothermus thermophilus 15.8 2 D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Mycothermus thermophilus D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Mycothermus thermophilus 15.8 D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 83000,SDS-PAGE Mycothermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GAII
-
 Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 55
-
-
 Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5.5
-
-
 Mycothermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Mycothermus thermophilus isoelectric focusing
-
 7.2