Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kianmehr, A.; Mahdizadeh, R.; Oladnabi, M.; Ansari, J.
    Recombinant expression, characterization and application of a dihydrolipoamide dehydrogenase with diaphorase activity from Bacillus sphaericus (2017), 3 Biotech, 7, 153 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.8.1.4 diagnostics Bacillus sphaericus DLD-diaphorase exhibits considerable potential to be used as a component of diagnostic tests for the quantification of metabolites Lysinibacillus sphaericus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 gene DLD, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 50000
-
 recombinant His-tagged enzyme, gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.4 protein N6-(lipoyl)lysine + NADH + H+ Lysinibacillus sphaericus
-
 protein N6-(dihydrolipoyl)lysine + NAD+
-
 r
1.8.1.4 protein N6-(lipoyl)lysine + NADH + H+ Lysinibacillus sphaericus PAD-91
-
 protein N6-(dihydrolipoyl)lysine + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Lysinibacillus sphaericus
-
-
-
1.8.1.4 Lysinibacillus sphaericus PAD-91
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 additional information DLD catalyzes the reduction of NAD+ by dihydrolipoamide and exhibits NADH-dependent diaphorase activity Lysinibacillus sphaericus ?
-
 ?
1.8.1.4 additional information DLD catalyzes the reduction of NAD+ by dihydrolipoamide and exhibits NADH-dependent diaphorase activity Lysinibacillus sphaericus PAD-91 ?
-
 ?
1.8.1.4 protein N6-(lipoyl)lysine + NADH + H+
-
 Lysinibacillus sphaericus protein N6-(dihydrolipoyl)lysine + NAD+
-
 r
1.8.1.4 protein N6-(lipoyl)lysine + NADH + H+
-
 Lysinibacillus sphaericus PAD-91 protein N6-(dihydrolipoyl)lysine + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.4 monomer 1 * 50000, recombinant His-tagged enzyme, SDS-PAGE Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Lysinibacillus sphaericus
1.8.1.4 DLD
-
 Lysinibacillus sphaericus
1.8.1.4 More cf. EC 1.6.99.3 Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD dependent on Lysinibacillus sphaericus
1.8.1.4 NADH
-
 Lysinibacillus sphaericus