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Literature summary extracted from

  • Zhang, J.; ten Pierick, A.; van Rossum, H.M.; Seifar, R.M.; Ras, C.; Daran, J.M.; Heijnen, J.J.; Wahl, S.A.
    Determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae using shikimate dehydrogenase as sensor reaction (2015), Sci. Rep., 5, 12846.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.25 analysis usage of Escherichia coli shikimate dehydrogenase as sensor reaction for determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae, quantitative measurements of physiological variables in the cytosolic compartment by GC-MS/MS, cytosolic NADPH/NADP ratio in batch experiments, overview. The steady state sensor reaction based cytosolic free NADPH/NADP ratio is 15.6 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 gene aroE from Escherichia coli sstrain DH5alpha, recombinant overexpression in Saccharomyces cerevisiae strain CEN.PK 113-5D yielding strain CEN.PK-aroE Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.25 additional information steady state cytosolic free and whole cell NADPH/NADP ratio in different Saccharomycs cerevisiae strains, with or without recombinant expression of shikimate dehydrogenase from Escherichia coli, thermodynamics and kinetics, overview Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.25 cytosol occurs only in the cytosol Escherichia coli 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 shikimate + NADP+ Escherichia coli
-
 3-dehydroshikimate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Escherichia coli P15770
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.25 9.68
-
 recombinant CEN.PK-aroE strain expressing the enzyme from Eschericha coli, pH 7.0, 25°C Escherichia coli
1.1.1.25 19.9
-
 pH 9.0, 25°C, recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 additional information usage of recombinant shikimate dehydrogenase as sensor reaction for determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae, quantitative measurements of physiological variables in the cytosolic compartment by GC-MS/MS, cytosolic NADPH/NADP ratio in batch experiments, method, overview Escherichia coli ?
-
 ?
1.1.1.25 shikimate + NADP+
-
 Escherichia coli 3-dehydroshikimate + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 AroE
-
 Escherichia coli
1.1.1.25 SDH
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 25
-
 assay at Escherichia coli
1.1.1.25 30
-
 in vivo assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 7
-
 in vivo assay at Escherichia coli
1.1.1.25 9
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+ specific for Escherichia coli
1.1.1.25 NADPH specific for Escherichia coli

General Information

EC Number General Information Comment Organism
1.1.1.25 additional information modelling of steady state and dynamic fluxes into pentose phosphate pathway and the flux split ratio into glycolysis and pentose phosphate pathway in Saccharomyces recombinantly expressing Escherichia coli shikimate dehydrogenase, overview Escherichia coli