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Literature summary extracted from
- Expression, purification and functional characterization of a novel 3alpha-hydroxysteroid dehydrogenase from Pseudomonas aeruginosa (2015), Protein Expr. Purif., 115, 102-108.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.50 | sequence comparison and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.50 | Ca2+ | - |
Pseudomonas aeruginosa |  |
| 1.1.1.50 | CO2 | - |
Pseudomonas aeruginosa | |
| 1.1.1.50 | Cu2+ | 67% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.50 | additional information | - |
additional information | steady-state kinetics | Pseudomonas aeruginosa |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.50 | Fe3+ | activates | Pseudomonas aeruginosa | |
| 1.1.1.50 | K+ | activates | Pseudomonas aeruginosa | |
| 1.1.1.50 | Mn2+ | activates | Pseudomonas aeruginosa | |
| 1.1.1.50 | additional information | no effect by 0.5 mM of Mg2+, Ni2+, and Al3+ | Pseudomonas aeruginosa | |
| 1.1.1.50 | Na+ | activates | Pseudomonas aeruginosa | |
| 1.1.1.50 | Zn2+ | activates 1.75fold at 0.5 mM | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 32000 | - |
x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.50 | a 3alpha-hydroxysteroid + NAD+ | Pseudomonas aeruginosa | - |
a 3-oxosteroid + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.50 | Pseudomonas aeruginosa | Q9I1X3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.50 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.50 | a 3alpha-hydroxysteroid + NAD+ | - |
Pseudomonas aeruginosa | a 3-oxosteroid + NADH + H+ | - |
r | |
| 1.1.1.50 | cholate + NAD+ | - |
Pseudomonas aeruginosa | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.50 | ? | x * 32000, recombinant His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.50 | 3alpha-HSD | - |
Pseudomonas aeruginosa |
| 1.1.1.50 | 3alpha-hydroxysteroid dehydrogenase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 30 | - |
recombinant enzyme | Pseudomonas aeruginosa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 15 | 45 | optimum at 30°C, over 60% of maximal activity in the range of 25-45°C, with the temperature increasing from 30°C to 45°C, the enzyme activity gradually decreases to 77.55% of the optimum whereas with the temperature decreasing from 30°C to 15°C, the enzyme activity rapidly drops to 39.4% of the optimum | Pseudomonas aeruginosa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 40 | - |
purified recombinant His-tagged enzyme, stable up to | Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 8 | - |
recombinant enzyme | Pseudomonas aeruginosa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 4 | 11 | activity range, profile overview | Pseudomonas aeruginosa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.50 | NAD+ | - |
Pseudomonas aeruginosa | |
| 1.1.1.50 | NADH | - |
Pseudomonas aeruginosa | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.50 | physiological function | 3alpha-hydroxysteroid dehydrogenase catalyzes the oxidation of the 3-hydroxyl group of steroids. The enzymatic conversion is a critical step in the enzymatic assay of urinary sulfated bile acids | Pseudomonas aeruginosa |
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