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Literature summary extracted from

  • Graciet, E.; Hu, R.; Piatkov, K.; Rhee, J.; Schwarz, E.; Varshavsky, A.
    Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen (2006), Proc. Natl. Acad. Sci. USA, 103, 3078-3083.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.29 expressed in Escherichia coli KPS22 cells Vibrio vulnificus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.29 C18S inactive Vibrio vulnificus
2.3.2.29 Y170F the mutant loses 90% of wild type enzyme activity Vibrio vulnificus
2.3.2.29 Y205F the mutant loses 90% of wild type enzyme activity Vibrio vulnificus
2.3.2.29 Y58F the mutant loses 70-80% of wild type enzyme activity Vibrio vulnificus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.29 L-leucyl-tRNALeu + N-terminal L-aspartyl-[protein] Vibrio vulnificus
-
 tRNALeu + N-terminal L-leucyl-L-aspartyl-[protein]
-
 ?
2.3.2.29 L-leucyl-tRNALeu + N-terminal L-glutamyl-[protein] Vibrio vulnificus
-
 tRNALeu + N-terminal L-leucyl-L-glutamyl-[protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.29 Vibrio vulnificus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.29
-
 Vibrio vulnificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.29 L-leucyl-tRNALeu + N-terminal L-aspartyl-[protein]
-
 Vibrio vulnificus tRNALeu + N-terminal L-leucyl-L-aspartyl-[protein]
-
 ?
2.3.2.29 L-leucyl-tRNALeu + N-terminal L-glutamyl-[protein]
-
 Vibrio vulnificus tRNALeu + N-terminal L-leucyl-L-glutamyl-[protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.29 bacterial protein transferase
-
 Vibrio vulnificus
2.3.2.29 BPT
-
 Vibrio vulnificus
2.3.2.29 LD,E-transferase
-
 Vibrio vulnificus
2.3.2.29 Leu-conjugating aa-transferase
-
 Vibrio vulnificus