EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.122 | Ag+ | complete inhibition at 1 mM | Acinetobacter sp. | |
1.1.1.122 | Co2+ | complete inhibition at 1 mM | Acinetobacter sp. | |
1.1.1.122 | Cu2+ | complete inhibition at 1 mM | Acinetobacter sp. | |
1.1.1.122 | EDTA | - |
Acinetobacter sp. | |
1.1.1.122 | Hg2+ | complete inhibition at 1 mM | Acinetobacter sp. | |
1.1.1.122 | N-ethylmaleimide | - |
Acinetobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.122 | 1.7 | - |
NADP+ | at pH 8.0 and 37°C | Acinetobacter sp. | |
1.1.1.122 | 2.6 | - |
L-fucose | at pH 8.0 and 37°C | Acinetobacter sp. | |
1.1.1.122 | 2.7 | - |
NAD+ | at pH 8.0 and 37°C | Acinetobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 23000 | - |
gel filtration | Acinetobacter sp. |
1.1.1.122 | 25000 | - |
1 * 25000, SDS-PAGE | Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.122 | L-fucose + NADP+ | Acinetobacter sp. | 100% activity | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.122 | L-fucose + NADP+ | Acinetobacter sp. SA-134 | 100% activity | L-fuconolactone + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.122 | Acinetobacter sp. | - |
- |
- |
1.1.1.122 | Acinetobacter sp. SA-134 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.122 | DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography | Acinetobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 2.3 | - |
cell-free extract, at pH 8.0 and 37°C | Acinetobacter sp. |
1.1.1.122 | 217 | - |
after 94.3fold purification, at pH 8.0 and 37°C | Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.122 | D-arabinose + NADP+ | good substrate with 65% activity compared to L-fucose | Acinetobacter sp. | D-arabinolactone + NADPH + H+ | - |
? | |
1.1.1.122 | D-arabinose + NADP+ | good substrate with 65% activity compared to L-fucose | Acinetobacter sp. SA-134 | D-arabinolactone + NADPH + H+ | - |
? | |
1.1.1.122 | L-fucose + NAD+ | - |
Acinetobacter sp. | L-fuconolactone + NADH + H+ | - |
? | |
1.1.1.122 | L-fucose + NAD+ | - |
Acinetobacter sp. SA-134 | L-fuconolactone + NADH + H+ | - |
? | |
1.1.1.122 | L-fucose + NADP+ | good substrate | Acinetobacter sp. | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.122 | L-fucose + NADP+ | 100% activity | Acinetobacter sp. | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.122 | L-fucose + NADP+ | good substrate | Acinetobacter sp. SA-134 | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.122 | L-fucose + NADP+ | 100% activity | Acinetobacter sp. SA-134 | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.122 | L-galactose + NADP+ | L-galactose is a poor substrate with 11% activity compared to L-fucose | Acinetobacter sp. | ? | - |
? | |
1.1.1.122 | additional information | no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose | Acinetobacter sp. | ? | - |
? | |
1.1.1.122 | additional information | no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose | Acinetobacter sp. SA-134 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.122 | monomer | 1 * 25000, SDS-PAGE | Acinetobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.122 | L-fucose dehydrogenase | - |
Acinetobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 50 | - |
- |
Acinetobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 35 | - |
the enzyme is stable below 35°C for 60 min | Acinetobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 8 | - |
- |
Acinetobacter sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 6 | 10 | the enzyme is stable between pH 6.0 and 10.0 for 60 min | Acinetobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.122 | NAD+ | the enzyme acts on both NAD+ and NADP+ in similar manner | Acinetobacter sp. | |
1.1.1.122 | NADP+ | the enzyme acts on both NAD+ and NADP+ in similar manner | Acinetobacter sp. |